文献类型：期刊文献

中文题名：基于药材表征和色谱技术对铁棒锤药材质量标准提升的相关研究

英文题名：Study on Quality Standard Improvement of Aconitum pendulum Based on Medicinal Materials Characterization and Chromatographic Technique

作者：林丽[1];高素芳[1];陈红刚[1];刘立[1];晋玲[1];郭玉环[1]

第一作者：林丽

机构：[1]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2020

卷号：22

期号：11

起止页码：1804

中文期刊名：中国现代中药

外文期刊名：Modern Chinese Medicine

收录：CSTPCD

基金：中央本级重大增减支项目(2060302);甘肃省科技重大专项(17ZD2FA009);甘肃省教育厅项目(2016B-057);甘肃省科技计划基础研究创新群体(1606RJIA323);中央财政引导地方科技创新平台项目(2016-A-02)。

语种：中文

中文关键词：表征;色谱技术;铁棒锤;乌头碱

外文关键词：characterization;chromatographic technique;Aconitum pendulum;aconitine

摘要：目的:建立铁棒锤药材表征特性和其有效成分的色谱检测方法,为铁棒锤质量标准提升提供参考。方法:运用中药鉴别手段对野生和栽培铁棒锤性状形态表征、显微特征、薄层色谱进行定性鉴别;采用紫外-可见分光光度法、高效液相色谱法对其总乌头碱及乌头碱含量进行定量分析。结果:在外观形态上铁棒锤野生品比栽培品个体明显偏小,块根干瘪,而显微鉴别上两者差异较小;薄层色谱中,斑点清晰,分离度好,可用于快速鉴别;紫外分光光度法其总生物碱以乌头碱计在0.012~0.072 mg·mL-1与峰面积呈良好的线性关系。高效液相色谱法在0.025~0.500 mg·mL-1与峰面积呈良好线性关系。不同批次铁棒锤中乌头碱与总乌头碱含量差异较大,野生品中的总乌头碱高于栽培品;子根中的乌头碱高于块根。结论:为确保临床用药安全,在铁棒锤入药时应区分野生品与栽培品、子根与块根。应综合考量药材各因素与品质之间的关系,建立多种有效且科学的质量评价方法。为其在质量评价中的进一步应用提供参考。
Objective:To establish the methods for characterization and active component detection of Aconitum pendulum,and to provide scientific basis for the promotion of the quality standard of A.pendulum.Methods:The morphological characters,microscopic characteristics and thin-layer chromatography of wild and cultivated A.pendulum were identified by means of the traditional Chinese medicine identification methods.The contents of total aconitine and aconitine were quantitatively analyzed by modern detection methods,namely ultraviolet-visible spectrophotometry and high performance liquid chromatography.Results:In terms of morphology,the wild A.pendulum was obviously smaller than the cultivated A.pendulum,and the roots were shriveled,but the difference between the two was small in microscopic identification.The thin-layer chromatography results showed that the spots were clear and well separated,which can be used for rapid identification.The total alkaloids detected by UV method were calculated as aconitine at 0.012-0.072 mg·mL-1,showing a good linear relationship.There was a good linear relationship between aconitine and peak area in the range of 0.025-0.500 mg·mL-1 in the HPLC method.The contents of total aconitine and aconitine in different batches of A.pendulum were significantly different,and the contents of aconitine in wild A.pendulum were higher than that in cultivated A.pendulum.Conclusion:In order to ensure the safety of clinical medication,wild and cultivated A.pendulum should be distinguished.The relationship between indexes and quality of A.pendulum should be considered comprehensively,and a variety of effective and scientific quality evaluation methods should be established to provide reference for further application in quality evaluation.