文献类型：期刊文献

英文题名：The role of miR-10b-5p in prostate cancer and its exosome-mediated angiogenesis effect

作者：Wang, Jia[1];Zhou, Chuan[2];Wang, Qi-dong[1];Zhang, Wen-bo[1];Wang, Chao[3];Zhang, Yun-Feng[3];Lv, Hao-Xuan[3];Zhou, Feng-Hai[1,4]

第一作者：王佳

通信作者：Zhou, FH[1]

机构：[1]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[2]Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Dept Geriatr Gen Surg, Chengdu 610072, Sichuan, Peoples R China;[3]Lanzhou Univ, Clin Med Coll 1, Lanzhou, Peoples R China;[4]Gansu Prov Hosp, Dept Urol, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), 35 Dingxi East Rd, Lanzhou, Gansu, Peoples R China.

年份：2025

卷号：298

起止页码：27

外文期刊名：CANCER GENETICS

收录：;Scopus(收录号：2-s2.0-105014616212);WOS:【SCI-EXPANDED(收录号:WOS:001567889200001)】；

基金：Scientific Research Foundation of Gansu Provincial People's Hospital (23GSSYD-12)Natural Science Foundation of Gansu Province "excellent doctoral program" (No. 24JRRA613)Study on synergistic effect of targeted delivery of dictinine and exosomes in the treatment of prostate cancer (24GSSYE-7)Construction of prostate cancer early warning model and its application based on AI multimodal imaging and liquid biopsy (25JRRA318)

语种：英文

外文关键词：Prostate cancer; miR-10b-5p; ZMYND11; Exosomal; Angiogenesis

摘要：Objective: Prostate cancer (PCa) continues to be a major cause of cancer-related mortality globally, underscoring the critical need for novel therapeutic strategies. This study investigates the oncogenic function of miR-10b-5p in PCa progression and evaluates its potential as both a diagnostic marker and therapeutic target. Methods: miR-10b-5p was initially identified as a candidate oncogene through bioinformatic analysis of The Cancer Genome Atlas (TCGA) PCa data, followed by validation of its expression levels in clinical PCa specimens via fluorescence in situ hybridization (FISH). Functional assays (proliferation, migration, invasion) were performed to assess the impact of miR-10b-5p on PCa cell behavior. The tumor suppressor ZMYND11 was confirmed as a direct target of miR-10b-5p using dual-luciferase reporter assays. The pro-angiogenic capacity of PCaderived exosomes harboring miR-10b-5p was evaluated using in vitro endothelial tube formation assays and in vivo mouse models. Results: miR-10b-5p expression was significantly elevated in PCa tissues and cell lines, and its levels correlated with aggressive tumor features. Mechanistically, miR-10b-5p directly suppressed ZMYND11 expression, thereby promoting PCa cell proliferation, migration, and invasion. Crucially, exosomes derived from miR-10b-5p-expressing PCa cells exhibited potent pro-angiogenic activity, significantly enhancing endothelial tube formation in vitro and stimulating tumor neovascularization in vivo. Conclusion: This study demonstrates that miR-10b-5p promotes prostate cancer progression by targeting ZMYND11, while its exosomes additionally exhibit pro-angiogenic effects, providing a novel therapeutic target for clinical intervention.