文献类型：期刊文献

英文题名：Deciphering the function of Xiangsha-Liujunzi-Tang in enhancing duodenal mucosal barrier by inhibiting MC/Tryptase/PAR-2 signaling pathway in functional dyspepsia rats

作者：Bai, Min[1,2];Zhao, Linna[1,2];Liu, Mengya[1];Li, Runfa[1];Yang, Yuping[1];Zhang, Yugui[1];Yuan, Xiaomei[1];Li, Yarong[3];Duan, Yongqiang[3,4];An, Yaorong[1];Cheng, Yingxia[3,4]

第一作者：Bai, Min

通信作者：Duan, YQ[1];Cheng, YX[1]

机构：[1]Gansu Univ Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Lab Anim Ind Technol Ctr, Lanzhou 730000, Gansu, Peoples R China;[3]Ningxia Med Univ, Coll Tradit Chinese Med, Yinchuan 750004, Ningxia, Peoples R China;[4]1160 Shengli St, Yinchuan City, Ningxia Hui Aut, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), 1160 Shengli St, Yinchuan City, Ningxia Hui Aut, Peoples R China.

年份：2024

卷号：319

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85172324096);WOS:【SCI-EXPANDED(收录号:WOS:001095542600001)】；

基金：Acknowledgments This project was supported by the Key R&D Special Project of Ningxia Hui Autonomous Region Science and Technology Department (2022YCZX0105) and the Graduate Student Innovation and Entrepre-neurship Project of Traditional Chinese Medicine of Gansu University in 2022 (2022CX06) .

语种：英文

外文关键词：Xiangsha-Liujunzi-Tang; Functional dyspepsia; Duodenal mucosal barrier; MC/Tryptase/PAR-2 signaling pathway

摘要：Ethnopharmacological relevance: Xiangsha-Liujunzi-Tang (XSLJZT) is a classical formula for treating the diseases of digestive system, which can effectively and significantly improve the symptoms of functional dyspepsia (FD) patients. The main function of XSLJZT is to benefit Qi and spleen, and harmonize stomach.Aim of the study: The purpose of this study was to investigate the intervention effect of XSLJZT on duodenal mucosal injury in FD rats and the response mechanism of MC/Tryptase/PAR-2 signal pathway. Materials and methods: Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) was employed to qualitatively and quantitatively identify the chemical component of XSLJZT. A comprehensive modeling method (iodoacetamide infusion + irregular diet + swimming exhaustion) was used to construct the FD rat model. XSLJZT decoction was given to intervene FD rats for 2 weeks. The indicators of digestive function including body mass, 3-h food intake, visceral sensitivity, gastric emptying rate and intestinal propulsion rate were routinely measured for FD rats. The pathological changes of duodenum and microstructure of intestinal epithelial cells were observed by HE staining and transmission electron microscopy respectively. The inflammatory factors (VCAM-1, IL-6, TNF-alpha, and ICAM-1) and histamine content were evaluated by enzymelinked immunosorbent assay (ELISA). The expression levels of Tryptase, PAR-2, ZO-1, beta-catenin, p-NF-kappa Bp65 and p-ERK1/2 in duodenal tissues were measured by Western blot (WB) and immunofluorescence colonystaining (IFC).Results: XSLJZT administration significantly improved the survival of FD rats, increased body mass and 3-h food intake, improved visceral sensitivity, and restored gastric emptying rate and intestinal propulsion rate. HE staining showed that XSLJZT recovered the structure of duodenal mucosal and reduced inflammatory infiltration. ELISA revealed that XSLJZT reduced the content of inflammatory factors (VCAM-1, IL-6, TNF-alpha, and ICAM1) and histamine. In addition, WB and IFC uncovered that the protein levels of ZO-1 and beta-catenin were upregulated and MC/Tryptase/PAR-2 signaling pathway was inhibited by XSLJZT.Conclusion: XSLJZT significantly improved the integrity of duodenal mucosa and decreased the inflammation in FD rats through the inhibition of MC/Tryptase/PAR-2 signaling pathway response.