文献类型：期刊文献

中文题名：聚酰胺树脂分离纯化藏药湿生扁蕾总黄酮的工艺研究

英文题名：Technical study on separation and purification of total flavonoid in Gentianopsis paludosa(Mum.)Ma by polyamide resin

作者：王晶晶[1];景明[1,2];陈正君[1];陈晖[1]

第一作者：王晶晶

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2013

卷号：30

期号：3

起止页码：35

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

基金：甘肃省科技支撑计划项目(0804NKCA119-08)

语种：中文

中文关键词：湿生扁蕾;总黄酮;聚酰胺树脂;分离纯化

外文关键词：Gentianopsis paludosa （ Mum. ） Ma; total flavonoid ; polyamide resin ; separation and purification

摘要：目的考察聚酰胺树脂分离纯化藏药湿生扁蕾总黄酮的最佳工艺。方法 70%乙醇回流提取湿生扁蕾,提取液回收乙醇后上聚酰胺树脂柱吸附,用不同体积分数乙醇洗脱,比色法检测洗脱液总黄酮的含量。结果聚酰胺树脂能很好地吸附湿生扁蕾中的总黄酮,70%乙醇洗脱总黄酮的回收率为90.32%。聚酰胺树脂在适当处理后可重复利用,但随着重复利用次数的增加,聚酰胺树脂对黄酮的吸附力不断下降。结论湿生扁蕾提取物经聚酰胺树脂分离纯化能得到较纯的总黄酮,且工艺简便易行,得率高,为工业化生产提供了参考依据。
Objective To investigate the optimum process on separation and purification of total flavonoid in Gentianopsis paludosa （ Mum. ） Ma by polyamide resin. Methods Gentianopsis paludosa （ Mum. ） Ma was extracted by circulation reflux of 70% ethanol, the extracting solution was adsorbed on polyamide resin column after the etha- nol was recovered, then eluted with different concentrations of ethanol, detected the content of total flavonoid in elu- tions by colorimetric method. Results Polyamide resin could adsorb the total flavonoid in Gentianopsis paludosa （ Mum. ） Ma very well, the retrieve ratio of the total flavonoid was 90.32% by 70% ethanol, the content of the elu- tion was 71.21%. Polyamide resin could be utilized repeatedly after disposed properly, but the capacity of adsorp- tion was declining with the times was increasing. Conclusion dosa（ Mum. ）Ma can be found by separation and purification of can offer reference for industrialization production. The more pure total flavonoid in Gentianopsis palu- polyamide resin, and the craft is not complicated,it