文献类型：期刊文献

中文题名：基于LC-MS的干旱胁迫下党参代谢组学分析

英文题名：Metabolomics Analysis of Codonopsis pilosula Under Drought Stress Based on LC-MS

作者：原静静[1];孙晓琛[1];栗锦鹏[1];杜弢[1];王惠珍[1]

第一作者：原静静

机构：[1]甘肃中医药大学药学院,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2021

卷号：27

期号：23

起止页码：145

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金项目(81760683,81960683);国家现代农业产业技术体系建设项目(CARS-21);甘肃省教育厅“双一流”科研重点项目(GSSYLXM-05);甘肃省教育厅优秀研究生“创新之星”项目(2021CXZX-754)。

语种：中文

中文关键词：党参;干旱胁迫;代谢组;差异代谢物;代谢通路

外文关键词：Codonopsis pilosula;drought stress;metabolic group;differential metabolites;metabolic pathways

摘要：目的:通过对采收期党参根部代谢组测定,探索党参适应干旱的生物学机制。方法:利用非靶向代谢组学液相色谱-质谱法(LC-MS),通过多元统计分析、单变量统计分析和代谢通路富集分析,对党参干旱胁迫下差异代谢物进行筛选。结果:(1)轻度干旱胁迫(LD)与正常(CK)组显著差异代谢物274个,其中142个上调,132个下调;中度干旱胁迫(MD)与CK组显著差异代谢物284个,其中157个上调,127个下调;重度干旱胁迫(SD)与CK组显著差异代谢物317个,其中133个上调,184个下调。(2)差异代谢物被注释到京都基因与基因组百科全书(KEGG)数据库得到82条差异代谢通路,其中鞘脂类代谢为极显著富集的差异代谢通路(P<0.01),精氨酸和脯氨酸代谢、色氨酸代谢、丙氨酸代谢、半乳糖代谢、烟酸及烟酰胺代谢、半胱氨酸和蛋氨酸代谢、花生四烯酸代谢、亚麻酸代谢和甘油脂类代谢为显著富集的差异代谢物通路(P<0.05)。(3)对富集前后3个对比组的差异代谢物进行分类分析,发现主要集中在脂肪酰基、羧酸类及其衍生物、有机氧化物3类,其次在鞘脂类、吲哚类及其衍生物、有机氮化合物、甘油磷脂、嘧啶及其衍生物、类肽、甘油酯等类;与糖类相关的代谢物在党参干旱胁迫中表现为上调,脂类相关的代谢物脂肪酰基表现为下调,其余均表现为上调。结论:阐明了干旱胁迫下党参根中代谢物的变化,其中糖类和脂类相关代谢物是党参受干旱刺激后的主要产物,且此类物质可能是提高党参抵御干旱能力的主要原因,这为后期深入研究党参抗旱机制奠定基础。
Objective:The biological mechanism of Codonopsis pilosula adaptation to drought was explored by determining the root metabolome of C.pilosula during harvesting.Method:Non-targeted metabonomics LC-MS was used to screen differential metabolites by multivariate statistical analysis,univariate statistical analysis and metabolic pathway enrichment analysis.Result:(1) There were 274 metabolites in LD vs CK group,142 of which were up-regulated and 132 of which were down-regulated.There were 284 metabolites with significant difference in MD vs CK group,of which 157 were up-regulated and 127 were down-regulated.There were 317 metabolites with significant difference in SD vs CK group,of which 133 were up-regulated and184 were down-regulated.(2) Differential metabolites were annotated into kyoto Encyclopedia of Gene and Genomes(KEGG)database and 82 differential metabolic pathways were obtained,among which sphingolipids metabolism was significantly enriched(P<0.01).Metabolism of arginine and proline,tryptophan,alanine,galactose,nicotinic acid and nicotinamide,cysteine and methionine,arachidonic acid,linolenic acid and glycerides were significantly enriched in different metabolite pathways(P<0.05).(3) The metabolites of the three comparison groups before and after enrichment were classified and analyzed.It was found that they were mainly concentrated in fatty acyls group,carboxylic acid and derivatives,and organ oxygen compounds,followed by sphingolipids,indoles and derivatives,organonitrogen compounds,glycerophospholipids,pyridines and derivatives,peptidomimetics,glycerolipids and so on.In the drought stress of C.codonopsis,carbohydrate related metabolites were mainly up-regulated,lipid related metabolites were mainly down-regulated,and all other metabolites were up-regulated.Conclusion:The changes of metabolites in the roots of C.pilosula under drought stress were elucidated.carbohydrate and lipid-related metabolites were the main products of C.pilosula under drought stress,and these metabolites may be the main reason to improve the ability of C.pilosula to resis drought,which laid a foundation for further study on the mechanism of C.pilosula to resist drought.