文献类型：期刊文献

英文题名：Green and efficient method to acquire high-value phycobiliprotein from microalgal biomass involving deep eutectic solvent-based ultrasound-assisted extraction

作者：Li, Ke[1,3];Jiang, Chunxue[1];Han, Sang-Il[2,4];Kang, Seulgi[1];Chen, Jingyan[1];Won, Danbi[1];Kang, Yua[1];Bae, Boyeon[1];Choi, Yoon-E[2];Kim, Hyung Sik[1];Lee, Jeongmi[1]

第一作者：李楷;Li, Ke

通信作者：Lee, J[1]

机构：[1]Sungkyunkwan Univ, Sch Pharm, Suwon 16419, Gyeonggi Do, South Korea;[2]Korea Univ, Div Environm Sci & Ecol Engn, Seoul 02841, South Korea;[3]Gansu Univ Chinese Med, Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou 730000, Gansu, Peoples R China;[4]Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jeongeup 56212, Jeollabuk Do, South Korea

第一机构：Sungkyunkwan Univ, Sch Pharm, Suwon 16419, Gyeonggi Do, South Korea

通信机构：[1]corresponding author), Sungkyunkwan Univ, Sch Pharm, Suwon 16419, Gyeonggi Do, South Korea.

年份：2024

卷号：449

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20241515877489);Scopus(收录号：2-s2.0-85189705074);WOS:【SCI-EXPANDED(收录号:WOS:001223610000001)】；

基金：This study was financially supported by two research grants from the National Research Foundation of Korea (Nos. 2022R1A6A1A03054419 and RS-2023-00208174) . The authors would like to thank Ms. Xue Dong for providing technical assistance with the SEM measurements.

语种：英文

外文关键词：Deep eutectic solvents; Microalgae; Cell disruption; Extraction; Phycoerythrin

摘要：Phycoerythrin (PE) is a phycobiliprotein holding great potential as a high-value food colorant and medicine. Deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) was applied to extract B-PE by disrupting the resistant polysaccharide cell wall of Porphyridium purpureum. The solubility of cell wall monomers in 31 DESs was predicted using COSMO-RS. Five glycerol-based DESs were tested for extraction, all of which showed significantly higher B-PE yields by up to 13.5 folds than water. The DES-dependent B-PE extraction efficiencies were proposedly associated with different cell disrupting capabilities and protein stabilizing effects of DESs. The DES-based UAE method could be considered green according to a metric assessment tool, AGREEprep. The crude extract containing DES was further subjected to aqueous two-phase system, two-step ammonium sulfate precipitation, and ultrafiltration processes. The final purified B-PE had a PE purity ratio of 3.60 and a PC purity ratio of 0.08, comparable to the purity of commercial products.