文献类型：期刊文献

中文题名：基于有效成分和近红外光谱研究不同施肥水平对藏药铁棒锤质量的影响

英文题名：Effect of Different Fertilization Levels on the Quality of Tibetan Medicine Aconitum pendulum Based on Active Components and Near Infrared Spectroscopy

作者：陈红刚[1];郭鸿儒[2];杜弢[1];侯嘉[1]

第一作者：陈红刚

机构：[1]甘肃中医药大学药学院/甘肃省中药质量与标准研究重点实验室培育基地,甘肃兰州730020;[2]兰州工业研究院,甘肃兰州730050

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）|甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2021

卷号：44

期号：12

起止页码：2752

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；PubMed;

基金：兰州市科技计划项目(2016-3-116);现代农业产业技术体系建设专项基金项目(CARS-21);甘肃省中药质量与标准研究重点实验室培育基地项目(ZYZL16-003);甘肃道地药材产业关键技术及其组方的基础与开发应用研究项目(GSSYLXM-05)

语种：中文

中文关键词：铁棒锤;施肥处理;乌头碱;近红外光谱;化学计量学

外文关键词：Aconitum pendulum Busch;Fertilization treatment;Aconitine;Near infrared spectroscopy;Chemometrics

摘要：目的:在大田条件下,研究不同施肥水平对铁棒锤质量的影响,为铁棒锤的广泛种植、科学施肥及标准化栽培提供指导依据。方法:采用“3414”法进行铁棒锤大田肥效试验,应用HPLC测定主要有效成分乌头碱的含量,近红外漫反射法测定不同配比氮磷钾施肥的铁棒锤药材的近红外光谱,并通过方差分析、聚类分析和主成分分析进行质量评价。结果:氮、磷、钾肥均对铁棒锤中乌头碱含量有极显著影响(P<0.001),且氮磷钾配施中磷肥占比高的,铁棒锤中乌头碱含量较高,效果较好。聚类分析和主成分分析结果表明,P_(2)O_(5)≥580.50 kg/hm^(2)时,施肥处理与铁棒锤药材质量具有较好的相关性。结论:建议最佳施肥量的磷肥(P_(2)O_(5)≥580.50 kg/hm^(2))可以有效提高铁棒锤中乌头碱的含量,通过“3414”田间施肥设计,说明科学有效施肥能提高铁棒锤的质量。
Objective:To study the effect of different fertilization levels on the quality of Aconitum pendulum in field,so as to provide guidance for extensive planting,scientific fertilization and standardized cultivation of Aconitum pendulum.Methods:The"3414"method was used to carry out the fertilizer effect test of Aconitum pendulum.HPLC was used to determine the content of the main effective ingredient aconitine,near infrared diffuse reflectance method was used to determine the near infrared spectrum of Aconitum pendulum with different proportions of nitrogen,phosphorus and potassium fertilization.The quality evaluation was carried out by analysis of variance,cluster analysis and principal component analysis.Results:The content of aconitine in Aconitum pendulum was significantly affected by nitrogen,phosphorus and potassium fertilization(P<0.001),and the combination of nitrogen,phosphorus and potassium fertilization with a high proportion of phosphorus fertilizer had a higher content of aconitine in Aconitum pendulum,and the effect was better.The results of cluster analysis and principal component analysis showed that when P_(2)O_(5)≥580.50 kg/hm^(2),there was a good correlation between fertilization and the quality of Aconitum pendulum.Conclusion:It is suggested that the best phosphate fertilizer(P_(2)O_(5)≥580.50 kg/hm^(2))can effectively improve the content of aconitine in Aconitum pendulum.The"3414"field fertilization design shows that scientific and effective fertilization can improve the quality of Aconitum pendulum.