文献类型：期刊文献

英文题名：CircRTN4 inhibits the progression of gastric cancer by sponging miR-424-5p and regulating LATS2 expression

作者：Li, Xiaoli[1,2];Wang, Tianming[1];Chen, Qian[1];Fu, Qi[1];Cui, Yan[1];Pan, Haibang[1]

第一作者：李旭立;Li, Xiaoli;李向丽;李小林

通信作者：Pan, HB[1]

机构：[1]Gansu Univ Chinese Med, Clin Med Sch 1, Lanzhou 730000, Peoples R China;[2]Gansu Prov Matern & Child Care Hosp, Lanzhou 730050, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Med Sch 1, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：22

期号：5

起止页码：967

外文期刊名：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

收录：;Scopus(收录号：2-s2.0-85166350035);WOS:【SCI-EXPANDED(收录号:WOS:001007498900005)】；

基金：Acknowledgements This work was supported by University Research Projects in Gansu Province (no. BH2012-021) and Gansu Province outstanding graduate students' "Innovation Star" project.

语种：英文

外文关键词：Gastric cancer; Circular RNA; miRNA

摘要：Purpose: To determine the expression of circRTN4 in gastric cancer (GC) and its role in tumor progression.Methods: GEO dataset GSE93541 was analyzed using GEO2R. Starbase website was used to predict the combination of miRNA and circRTN4. The relationship between circRTN4 and prognosis was analyzed using Kaplan-Meier Plotter database, while expression levels of circRTN4, miR-424-5p, and LATS2 were assessed by quantitative real time-polymerase chain reaction (qRT-PCR). CCK8, EDU, Transwell, and Western blot were used to assess GC proliferation, migration, invasion, and stemness. Lastly, co-transfection of miR-424-5p or si-LAST2 was reversely used to demonstrate the regulatory effect of circRTN4 on the progression of gastric cancer cells. Significantly downregulated circRTN4 in GC was screened, and the combined miR-424-5p and downstream gene LATS2 were predicted by Starbase.Results: The average relative expression of circRTN4 mRNA in GC tissues was significantly lower than in adjacent tissues. MiR-424-5p was highly expressed in tumor tissues, while LATS2 decreased (p < 0.05). Low expression of circRTN4 was associated with a low survival rate in patients. pLCDH-circRTN4 significantly inhibited the proliferation of gastric cancer cells (p < 0.05). Overexpression of circRTN4 inhibited the migration and invasion of tumor cells, while pLCDH-circRTN4 reduced the ability of GC stem cells and expressions of MMP2 and OCT4.Conclusion: Expression of circRTN4 decreases in GC, and contributes to the development and progression of this disease by increasing the levels of LATS2 and binding with miR-424-5p. This suggests that circRTN4 may serve as a promising prognostic marker as well as a potential therapeutic target for gastric cancer.