文献类型：期刊文献

英文题名：Cytotoxicity of two water-soluble polysaccharides from Codonopsis pilosula Nannf. var. modesta (Nannf.) LTShen against human hepatocellular carcinoma HepG2 cells and its mechanism

作者：Bai, Ruibin[1];Li, Wuyan[2];Li, Yingdong[3];Ma, Ming[1];Wang, Yanping[1];Zhang, Jing[1];Hu, Fangdi[1]

第一作者：Bai, Ruibin

通信作者：Hu, FD[1]

机构：[1]Lanzhou Univ, Sch Pharm, 199 Dong Gang Rd West, Lanzhou 730000, Gansu, Peoples R China;[2]Temple Univ, Sch Med, Ctr Inflammat Translat & Clin Lung Res, Philadelphia, PA 19122 USA;[3]Gansu Univ Tradit Chinese Med, Inst Integrated Tradit Chinese & Western Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Pharm, 199 Dong Gang Rd West, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharm, 199 Dong Gang Rd West, Lanzhou 730000, Gansu, Peoples R China.

年份：2018

卷号：120

起止页码：1544

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000449892800023)】；

基金：This study was supported by the Projects of Science and Technology Agency of Gansu (No. 1504FKCA010) and Science and Technology Agency of Lanzhou (Nos. 2016-2-8 and 2017-4-119) and the major projects of Science and Technology Department of Gansu Province (No. 17ZD2FA009).

语种：英文

外文关键词：Codonopsis pilosula; Polysaccharides; Anti-tumor activity

摘要：Two water-soluble polysaccharides named CPP1a and CPP1c were isolated from C. pilosula Nannf. var. modesta L.T.Shen by hot-water extraction and purified by graded alcohol precipitation and DEAE-52 cellulose column. The structure of CPP1c with higher yield has been characterized while its antitumor activities has not been elucidated. In this study, we firstly analyzed the chemical structure of CPP1a. The results of instrumental analysis combined with chemical analysis showed that CPP1a was composed of -> 1)-beta-L-Rhap-(4 ->, -> 1)-beta-Arap-(5 ->, -> 1)-beta-D-GalpA-(4 ->, -> 1)-beta-D-Galp-(6 ->, terminal-beta-D-Glcp in a molar ratio of 1:12:1:10:3 and its relative and absolute molecular weight were 1.01 x 10(5) Da and 1.03 x 10(5) Da respectively. Further, the cytotoxicity assay indicated that CPP1a and CPP1c were more sensitive to HepG2 cells than cervical carcinoma Hela cells and gastric carcinoma MKN45 cells. Both of CPP1a and CPP1c could influence cell morphology, inhibit the migration and induce apoptosis by affecting the G2/M phase of HepG2 cells. Preliminary mechanism studies confirmed that CPP1a and CPP1c could induce apoptosis through up-regulating the ratio of Bax/Bcl-2 and activating caspase-3. According to previous research, we might speculate that the reason for the stronger cytotoxicity and pro-apoptotic effect of CPP1c than that of CPP1a can be attributed to its high uronic acid content. (C) 2018 Elsevier B.V. All rights reserved.