文献类型：期刊文献

中文题名：Exploring the regulatory mechanism of“Tiao Qi Ming Mu”point prescription on visual plasticity in monocular vision deprivation rats based on cAMP/PKA pathway

英文题名：基于cAMP/PKA通路探讨“调气明目”穴方对单眼形觉剥夺大鼠视觉可塑性的调节机制

作者：LIU Anguo[1];ZHANG Qian[1];ZHOU Na[2];KAN Lili[3];HAO Xiaolu[1];WU Yanhui[1];ZHENG Xiaolin[1];YAN Xingke[1]

第一作者：刘安国

机构：[1]School of Acupuncture and Tuina,Gansu University of Chinese Medicine,Lanzhou 730000,China;[2]The First People’s Hospital of Huangzhong District,Xining City,Qinghai Province,Xining 810000,China;[3]Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730000,China

第一机构：甘肃中医药大学针灸推拿学院

年份：2025

卷号：23

期号：6

起止页码：476

中文期刊名：Journal of Acupuncture and Tuina Science

外文期刊名：针灸推拿医学(英文版)

基金：国家自然科学基金项目,No.82260965,No.81260560;甘肃省自然科学基金项目,No.22JR5RA583;甘肃中医药大学“岐黄英才”导师专项基金,No.ZYXKSD-202220,No.ZYXKBD-202208.

语种：英文

中文关键词：Acupuncture Therapy;Amblyopia;Vision Deprivation;Visual Cortex;Metabotropic Glutamate Receptors;cAMP/PKA Signal Pathway;Rats

外文关键词：针刺疗法;弱视;形觉剥夺;视皮层;代谢性谷氨酸受体;cAMP/PKA信号通路;大鼠

摘要：Objective:To investigate the intervention mechanism of acupuncture on visual cortical plasticity in rats with monocular vision deprivation by examining the regulatory effects of“Tiao Qi Ming Mu”(Qi-regulating and eyesight-improving)on the metabotropic glutamate receptor 1(mGluR1)-mediated cyclic-adenosine monophosphate(cAMP)/protein kinase A(PKA)signaling cascade.Methods:Forty-eight Sprague-Dawley rats were randomly divided into a blank group,a model group,a covering group,and an acupuncture group.The deprivation amblyopia model was established by right eyelid suture in all groups except the blank group.In the covering group,the sutured right eye was cut open,and the left eyelid was sutured on the 7th day after modeling.In the acupuncture group,the“Tiao Qi Ming Mu”point prescription was administered starting on the 7th day after model establishment and continued for 14 d.The electrophysiological changes in binocular vision of rats were detected using pattern visual evoked potential(P-VEP);in visual cortex area 17,the mGluR1 protein expression was detected using immunofluorescence staining,the mGluR1 mRNA level was detected by real-time polymerase chain reaction,and the mGluR1,cAMP,and PKA protein levels were detected by Western blotting.Results:Compared to the blank group,the P-VEP’s P100 wave latency period delayed,and the amplitude reduced in the vision-deprived eye of the model group,showing statistical significance(P<0.05);the protein and mRNA expression of mGluR1 and the protein expression of cAMP and PKA decreased in visual cortex area 17 in the model group,and the differences were significant(P<0.05).After 14 d of intervention,compared to the model group,the vision-deprived eye’s P-VEP waveform improved with advanced P100 wave latency periods and increased amplitudes in the covering group and the acupuncture group,and the differences were significant(P<0.05);the fluorescence expression of mGluR1 protein and the protein and mRNA expression of mGluR1 increased significantly(P<0.05);the cAMP and PKA protein expression increased significantly(P<0.05)in the covering group and the acupuncture group.Conclusion:“Tiao Qi Ming Mu”point prescription can activate the cAMP/PKA signaling pathway mediated by mGluR1 in visual cortex area 17 and promote the synaptic plasticity changes of visual cortex neurons of monocular vision-deprived amblyopia model rats,thereby improving the optic nerve conduction efficiency and promoting the recovery of visual function.
目的:观察“调气明目”穴方对代谢性谷氨酸受体1(mGluR1)介导的环腺苷酸(cAMP)/蛋白激酶A(PKA)通路的级联反应过程的调节效应,探讨针刺对单眼形觉剥夺大鼠视皮层可塑性的干预机制。方法:将48只Sprague-Dawley大鼠随机分为空白组、模型组、遮盖组和针刺组。除空白组外其余各组均采用右眼睑缝合的方法建立剥夺性弱视模型。遮盖组于造模后第7 d剪开右眼缝合处,缝合左侧眼睑。针刺组于造模后第7 d针刺“调气明目”穴方,干预14 d。通过图形视觉诱发电位(P-VEP)检测大鼠双眼视觉电生理变化;免疫荧光染色法检测视皮层17区mGluR1蛋白表达情况,借助实时聚合酶链反应检测mGluR1 mRNA含量,免疫印迹法检测mGluR1、cAMP及PKA蛋白含量。结果:与空白组相比,模型组大鼠剥夺眼P-VEP P100波潜伏期明显延迟,振幅显著降低,差异均有统计学意义(P<0.05);视皮层17区mGluR1蛋白及mRNA,cAMP及PKA蛋白表达显著降低,差异均有统计学意义(P<0.05)。干预14 d后,与模型组相比,遮盖组和针刺组大鼠剥夺眼P-VEP波形均有显著改善,P100波潜伏期明显提前,振幅明显升高,差异均有统计学意义(P<0.05);mGluR1蛋白荧光表达、mGluR1蛋白及mRNA的表达均显著升高(P<0.05);cAMP及PKA蛋白表达显著升高(P<0.05)。结论:“调气明目”穴方可以激活单眼剥夺弱视模型大鼠视皮层17区mGluR1介导的cAMP/PKA信号通路,促进视皮层神经元突触可塑性改变,从而提高视神经传导效率,促进视功能恢复。