文献类型：期刊文献

中文题名：超高效液相色谱法对铁棒锤药材指纹图谱研究

英文题名：Establishment of Fingerprints of Aconitum Flarum Hand. Mazz by UPLC

作者：林丽[1,2];高素芳[1];晋玲[2];陈红刚[1];韦雪玲[1]

第一作者：林丽

机构：[1]甘肃中医药大学,兰州730000;[2]中(藏)药资源研究所,兰州730000

第一机构：甘肃中医药大学

年份：2017

卷号：52

期号：10

起止页码：865

中文期刊名：中国药学杂志

外文期刊名：Chinese Pharmaceutical Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；PubMed;

基金：国家中医药管理局中医药行业专项甘肃省代表性区域中药资源保护利用资助项目(201207002-02);甘肃省科技计划基础研究创新群体"甘肃省中藏药资源评价;保护与开发利用"资助项目(1606RJIA323);中央财政引导地方科技创新平台项目"甘肃省中药资源与新产品开发平台建设"资助项目(2016-A-02)

语种：中文

中文关键词：超高效液相色谱法;铁棒锤;指纹图谱

外文关键词：UPLC ; Aconitum Flarum Hand. Mazz ; fingerprint

摘要：目的建立超高效液相色谱法测定铁棒锤药材的指纹图谱。方法采用ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×50mm,1.7μm);以0.2%甲酸水0.05%TFA(A)-甲醇(B)-乙腈(C)为流动相,进行梯度洗脱;流速为0.3 mL·min^(-1),柱温35℃,检测波长275 nm,进样量1.000μL。结果建立了铁棒锤药材的UPLC特征指纹图谱共有模式,标定了14个共有峰,并指认了其中2个主成分峰,13批次铁棒锤药材的相似度为0.850~0.980,栽培品与野生品之间存在差异。结论该方法精密度、稳定性及重复性较好,特征性及专属性强,建立的UPLC指纹图谱为鉴别铁棒锤的野生品和栽培品以及质量控制提供科学方法。
OBJECTIVE To establish the fingerprint of Aconitum Flarum Hand. Mazz Busch. METHODS UPLC analysis was performed on ACQUITY UPLC BEH C1s column（2.1 mm×50mm,1.7μm）;. Gradient elution was conducted. Mobile A was 0. 2% formic acid and 0. 005 % TFA in water; mobile B was methanol; and mobile C was acetonitrile. The flow rate was 0. 3 mL·min^-1. The detection was carried out at 275 nm. The column temperature was maitained at 35℃. The injection volume was 1.0μL. RESULTS The UPLC fingerprint of Aconitum Flarum Hand. Mazz was established, and 14 common peaks were found including the peaks of two major constituents. The similarity factors ranged from 0. 850 to 0. 980. In addition, there was significant difference between wild and cultivated samples. CONCLUSION This UPLC method has satisfactory precision, stability, repeatability and speci-ficity. It provides a scientific method for identifying wild and cultivated Aconitum Flarum Hand. Mazz using fingerprint.