文献类型：期刊文献

中文题名：化浊颗粒对2型糖尿病大鼠磷脂酰肌醇3激酶和葡萄糖转运蛋白4基因表达的影响

英文题名：Effects of Huazhuo Granule on Expressions of PI-3K and GLUT4 mRNA in Rats with Type 2 Diabetes

作者：康学东[1];李菲[2];杨维杰[1];余臣祖[2]

第一作者：康学东

机构：[1]甘肃中医药大学附属医院;[2]甘肃中医药大学

第一机构：甘肃中医药大学第二附属医院

年份：2015

卷号：22

期号：7

起止页码：67

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省教育厅研究生导师科研项目计划(1206-06)

语种：中文

中文关键词：化浊颗粒;2型糖尿病;胰岛素抵抗;磷脂酰肌醇3激酶;葡萄糖转运蛋白4;大鼠

外文关键词：Huazhuo Granule;type 2 diabetes;insulin resistance;PI-3K;rats

摘要：目的观察化浊颗粒对2型糖尿病(T2DM)大鼠磷脂酰肌醇3激酶(PI-3K)、葡萄糖转运蛋白4(GLUT4)在骨骼肌组织中表达的影响,探讨其对PI-3K及GLUT4信号通路激活的作用。方法采用高脂饲料喂养联合小剂量链脲佐菌素腹腔注射建立T2DM大鼠模型,随机分为化浊颗粒组、罗格列酮组、模型组。各给药组给予相应药物灌胃,模型组与空白组予生理盐水灌胃,观察大鼠一般状态、体质量及摄食量。干预10周后,予10%水合氯醛麻醉大鼠,检测大鼠空腹血糖(FBG)、葡萄糖耐量试验餐后2 h血糖(2 h PG)、空腹胰岛素(FINS)水平;RT-PCR检测各组大鼠骨骼肌组织PI-3K和GLUT4 m RNA表达的水平。结果与模型组比较,化浊颗粒组与罗格列酮组大鼠FBG、2 h PG及FINS水平降低(P<0.05),PI-3K及GLUT4 m RNA表达水平升高(P<0.05)。结论化浊颗粒具有提高胰岛素敏感性的作用,与激活骨骼肌组织胰岛素信号传导通路中PI-3K和GLUT4 m RNA的表达有关。
Objective To observe the effects of Huazhuo Granule on expression of phosphatidylinositol-3-kinase （PI-3K） and glucose transporter 4 （GLUT4） in skeletal muscle type 2 diabetes （T2DM） rats;To discuss its effects on activating signal path of PI-3K and GLUT4. Methods High-fat diet combined with low-dose streptozotocin intraperitoneal injection was used to establish T2DM rat models. The rats were randomly divided into blank group, Huazhuo Granule group, rosiglitazone group, and model group. Each treatment group was given relevant medicine for gavage. Model group and blank control group were given normal saline for gavage. The general state, weight, and food ration of rats were observed. After ten-week medicine intervention, 10%chloral hydrate was used for anesthesia to detect the levels of FBG, OGTT （2 h PG）, and FINS. RT-PCR was used to detect the expressions of PI-3K and GLUT4 mRNA of rat skeletal muscle in all groups. Results Compared with model group, the levels of FBG, 2 h PG, and FINS decreased （P〈0.05）;the expressions of PI-3K and GLUT4 mRNA of rat skeletal muscle increased significantly （P〈0.05）. Conclusion Huazhuo Granule has the effects on increasing insulin sensitivity, which is related to activating insulin signal path of PI-3K and GLUT4 of skeletal muscle tissues.