文献类型：期刊文献

英文题名：Acute hypoxia induces sleep disorders via sima/HIF-1α regulation of circadian rhythms in adult Drosophila

作者：Wang, Shuwei[1,5];Zhou, Shihong[1];Jiang, Xiaolin[2,3];Yang, Dan[2,3];He, Jianzheng[2,3,4];Xiu, Minghui[1,4]

第一作者：Wang, Shuwei

通信作者：Xiu, MH[1];He, JZ[2]

机构：[1]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Prov level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Coll Basic Med, Lanzhou 730000, Peoples R China;[4]Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China;[5]Shandong Univ Dezhou Hosp, Dept Clin Lab, Dezhou Hosp, Qilu Hosp, Dezhou 253000, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Prov level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;

年份：2025

卷号：294

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-86000746073);WOS:【SCI-EXPANDED(收录号:WOS:001447981400001)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 82004228) , Gansu High Education Innovation Ability Improvement Project (No. 2023A-086) , Foundation from the Collaborative Innovation Center for Traditional Chinese Medicine Prevention and Control of Nutrition and Environment Related Diseases in Northwest China (No. ZYXT-24-06) , Foundation from Key Laboratory of Dunhuang Medicine and Translational Education Ministry of Education (No. DHYX24-02) .

语种：英文

外文关键词：Acute hypoxia; Circadian rhythms; Sleep disorders; Drosophila

摘要：The atmospheric oxygen concentration is significantly reduced in highland regions compared to lowland areas. The first entering the plateau can induce sleep disorders in individuals, primarily attributed to insufficient oxygen supply. This study used Drosophila melanogaster as a model organism to better understand the molecular mechanism of acute hypoxia-induced sleep disorders. The Drosophila activity monitoring system (DAMS) was employed to observe the sleep-wake in adult (w1118, simaKG07607, and clockjrk) female flies. Quantifying the relative mRNA expression levels of sima and circadian clock genes in the head of flies was accomplished by utilizing qRT-PCR. Acute hypoxia caused sleep disorders in w1118 flies, such as shortened sleep duration and length, and prolonged sleep latency. PCR results showed that sima and clock genes were up-regulated in ZT6 and ZT12 and down-regulated in ZT0 and ZT18 in acute hypoxic w1118 flies compared to normoxic w1118 flies. Under normoxic conditions, sleep indexes in simaKG07607 flies were not substantially different from w1118 flies. However, clockjrk flies demonstrated a reduced sleep duration, decreased sleep bout length, and increased sleep latency and activities. Sleep and gene expression in simaKG07607 flies under acute hypoxic conditions were not significantly different from those under normoxic conditions. Surprisingly, sleep and gene expression in clockjrk flies showed opposite trends to w1118 flies. The present study indicates that acute hypoxia disrupt circadian rhythms through the activation of sima/HIF-1 alpha, leading to the onset of sleep disorders, with Clock signaling potentially serving as a contributing factor.