文献类型：期刊文献

中文题名：颊针对类风湿性关节炎家兔镇痛效应及脑脊液八肽胆囊收缩素和β-内啡肽的影响

英文题名：Analgesic Effect of Buccal Acupuncture and Changes of β-EP and CCK-8 in Cerebrospinal Fluid of Experimental Rheumatoid Arthritis Rabbits

作者：任超展[1];杜小正[1];方晓丽[1]

第一作者：任超展

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2012

卷号：19

期号：12

起止页码：37

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：甘肃省中医药科研立项课题(GZK-2010-60)

语种：中文

中文关键词：类风湿性关节炎;颊针;针刺镇痛;八肽胆囊收缩素;β-内啡肽;兔

外文关键词：rheumatoid arthritis; buccal acupuncture; acupuncture analgesia; CCK-8; β-EP; rabbits

摘要：目的观察颊针对类风湿性关节炎家兔的镇痛效应,并探讨其中枢作用机制。方法 48只青紫蓝兔随机分为正常组、模型组、体针组和颊针组,将后2组又随机分为针后即时(0 h)和针后1 h亚组,每组8只。采用卵蛋白诱导造成类风湿性关节炎模型。体针组针刺双侧"膝眼"和"足三里"1次,颊针组针刺双侧颊针"膝"1次。针刺后比较正常组、模型组、体针组和颊针组家兔关节局部痛阈及脑脊液中八肽胆囊收缩素(CCK-8)和β-内啡肽(β-EP)的含量。以K+导入法引起家兔腿收缩的最小电流强度作为痛阈;用放射免疫法测定β-EP、CCK-8的含量。结果模型组痛阈明显低于正常组(P<0.01);与模型组比较,体针组和颊针组痛阈均明显升高(P<0.01);颊针组0 h痛阈明显高于体针组0 h痛阈(P<0.01),颊针组1 h痛阈与体针组1 h痛阈比较无明显差异(P>0.05)。针刺后2组β-EP含量均升高,颊针组0 hβ-EP含量明显高于体针组0 hβ-EP含量(P<0.01),颊针组1 hβ-EP含量与体针组1 hβ-EP含量无明显差异(P>0.05);针刺后2组CCK-8含量均向正常水平恢复,颊针组0 h CCK-8含量明显高于体针组0 h CCK-8含量(P<0.05),颊针组1 h CCK-8含量与体针针组1 h CCK-8含量无明显差异。结论颊针即时镇痛效应优于体针,针刺促使脑脊液中β-EP含量升高和CCK-8含量向正常水平恢复,这可能是颊针镇痛中枢作用机制之一。
Objective To observe the analgesic effect of buccal acupuncture and explore its central analgesic mechanism in experimental rheumatoid arthritis rabbits. Methods A total of 48 rabbits were randomly divided into normal control group, model group, body-acupuncture group, buccal acupuncture group, and the later 2 groups were further randomized into 0 h and 1 h subgroups, with 8 cases in each group. The rheumatoid arthritis model was established by egg-albumin. For rabbits of body-acupuncture group, bilateral ＂Ydyan＂ （EX-LE5） and ＂Zusanli＂ （ST 36） were punctured for 1 min, with the needle retained for 30 min. For rabbits of buccal acupuncture group, acupuncture was applied to ＂xi＂ for 1 min, with the needle retained for 30min. The pain threshold was detected with K＋ import stimulation method, and β-EP and CCK-8 contents in cerebrospinal fluid were assayed with radioimmunoassay. Results Compared with the control group, the pain threshold of model group lowered significantly （P 〈0.01）. Compared with model group, the pain threshold of body-acupuncture group and buccal acupuncture group increased significantly （P 〈 0.01）. Compared with body-acupuncture group, the pain threshold of buccal acupuncture group at 0 h increased significantly （P〈O.01）. Both [3-EP and CCK-8 contents of buccal acupuncture group at 0 h were significantly higher than those of body-acupuncture group （P〈0.01, P〈 0.05）. There were no significant differences in contents of [3-EP and CCK-8 between buccal acupuncture group and body-acupuncture group. Conclusion The instant analgesic effect of buccal acupuncture is superior to body-acupuncture. Buccal acupuncture can effectively raise cerebral β-EP and CCK-8 contents, which may contribute to its central effect.