文献类型：期刊文献

中文题名：小黄姜药材超高效液相色谱指纹图谱研究

英文题名：UPLC Fingerprints of Globba Racemosa

作者：王咏梅[1];杨泳琳[2];苏蕊[2];江凯航[1,2];朱旭江[1,2]

第一作者：王咏梅

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃省药品检验研究院·国家药品监督管理局中药材饮片质量控制重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2026

卷号：35

期号：5

起止页码：92

中文期刊名：中国药业

外文期刊名：China Pharmaceuticals

基金：甘肃省药品监督管理局药品科研项目[2023GSMPA061]。

语种：中文

中文关键词：小黄姜;超高效液相色谱法;指纹图谱;质量评价

外文关键词：Globba racemosa;UPLC;fingerprints;quality evaluation

摘要：目的建立小黄姜药材的超高效液相色谱(UPLC)指纹图谱,并评价其质量。方法色谱柱为Waters CORTECS T_(3)柱(100 mm×2.1 mm,1.6μm),流动相为乙腈-水(梯度洗脱),流速为0.35 mL/min,检测波长为280 nm,柱温为35℃,进样量为1μL。以6-姜辣素峰为参照峰,建立14批样品的UPLC叠加指纹图谱,确定共有峰,并采用中药色谱指纹图谱相似度评价系统(2012版)进行相似度评价。结果共确定9个共有峰,相似度均大于0.94,共指认出峰2、峰3、峰5分别为6-姜辣素、8-姜酚、10-姜酚。结论所建立的UPLC指纹图谱可为小黄姜药材的质量评价提供参考。
Objective To establish an ultra-high performance liquid chromatography(UPLC)fingerprint of Globba racemosa,and to evaluate the quality of Globba racemosa.Methods The chromatographic column was Waters CORTECS T_(3) column(100 mm×2.1 mm,1.6μm),the mobile phase was acetonitrile-water(gradient elution),the flow rate was 0.35 mL/min,the detection wavelength was 280 nm,the column temperature was 35℃,and the injection volume was 1μL.With 6-gingerol peak as a reference peak,UPLC overlapped fingerprints of 14 batches of samples were established to identify common peaks,and similarity evaluation was performed by the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 Version).Results Nine common peaks were identified,with similarity>0.94,and peaks 2,3,and 5 were identified as 6-gingerol,8-gingerol,and 10-gingerol,respectively.Conclusion The established UPLC fingerprint can provide a reference for the quality evaluation of Globba racemosa medicinal material.