文献类型：期刊文献

英文题名：Changes in growth characteristics and secondary metabolites in Sinopodophyllum hexandrum with increasing age

作者：Zhao, Qiaozhu[1];Li, Meiling[1];Li, Mengfei[1];Jin, Ling[2];Wei, Jianhe[3]

第一作者：Zhao, Qiaozhu

通信作者：Li, MF[1]

机构：[1]Gansu Agr Univ, Coll Life Sci & Technol, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[3]Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

第一机构：Gansu Agr Univ, Coll Life Sci & Technol, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Life Sci & Technol, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China.

年份：2023

卷号：196

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20231013691481);Scopus(收录号：2-s2.0-85149436074);WOS:【SCI-EXPANDED(收录号:WOS:000952064600001)】；

基金：The work was funded by the National Natural Science Foundation of China (81560617) , Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003) , and earmarked fund for CARS (CARS -21) .

语种：英文

外文关键词：Gene expression; Growth characteristics; Plant age; Podophyllotoxin; Secondary metabolites; Sinopodophyllum hexandrum

摘要：Sinopodophyllum hexandrum is a perennial rhizomatous herb that produces the highly valued lignin podo-phyllotoxin (PPT). Although the effects of environmental factors on growth and secondary metabolites accu-mulation have been revealed, the changes with increasing plant age have not yet been conducted. Here, growth characteristics, contents of lignans and flavonoids, and expression levels of key genes in 2-to 9-year-old plants were systematically examined. The growth parameters significantly increased, and there were three distinctly morphological variants with single, two, and three leaves with increasing plant age. The accumulation of two lignans PPT and 4'-demethylepipodophyllotoxin (4'-DMEP), three target flavonoids, total flavonoids and phenolic, as well as in vitro antioxidant capacity maximized at 5-6 years. The expression levels of 8 key genes participating in lignan and flavonoid biosynthesis were up-regulated with increasing plant age. The findings provide the compelling evidence to guide sustainable cultivation and collection of S. hexandrum.