文献类型：期刊文献

中文题名：红景天苷对97H细胞增殖、迁移、侵袭及凋亡的影响

英文题名：Effects of salidroside on proliferation,migration,invasion and apoptosis of 97H cells

作者：蒋兵[1,2];杨韬[1];封龙飞[1];王涛[2];苏海翔[1,2]

第一作者：蒋兵

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃省医学科学研究院,甘肃省肿瘤医院,甘肃兰州730050

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2023

卷号：39

期号：3

起止页码：445

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；

基金：甘肃省肿瘤分子病理诊断临床医学研究中心项目(No 18JR2FA010);甘肃省中医药管理局课题(No GZKP-2021-21);甘肃省2021年度重点人才项目。

语种：中文

中文关键词：肝癌;红景天苷;增殖;迁移;侵袭;凋亡

外文关键词：liver cancer;salidroside;proliferation;migration;invasion;apoptosis

摘要：目的探究红景天苷对人高转移性肝癌细胞(97H细胞)增殖、迁移、侵袭和凋亡的影响。方法采用多功能细胞分析仪检测红景天苷对97H细胞增殖的影响,划痕实验检测红景天苷对97H细胞迁移能力的影响,Transwell小室实验检测红景天苷对97H细胞侵袭能力的影响,倒置显微镜观察红景天苷对97H细胞形态的影响,透射电镜观察红景天苷对97H细胞中线粒体的影响,流式细胞术检测红景天苷对97H细胞凋亡及周期分布的影响,q-PCR技术检测红景天苷对97H细胞中相关凋亡基因的影响,蛋白免疫印迹技术检测红景天苷对97H细胞相关迁移、侵袭及凋亡蛋白的影响。结果与空白组相比,红景天苷处理组对97H细胞的增殖、迁移及侵袭均具有一定的抑制作用,且诱导97H细胞凋亡。红景天苷可以上调Caspase-3基因的相对表达(P<0.05),且可以上调E-cad、Bax、Caspase-3及Caspase-9蛋白的相对表达(P<0.05),下调N-cad、Girdin及Bcl-2蛋白的相对表达(P<0.05)。结论红景天苷对97H细胞的增殖、迁移及侵袭均具有抑制作用,且通过线粒体途径诱导97H细胞凋亡。
Aim To study the effect of salidroside on the proliferation,migration,invasion and apoptosis of human highly metastatic liver cancer cells(97H cells).Methods Multifunctional cell analyzer was used to determine the effect of salidroside on the proliferation of 97H cells.Scratch assay was used to determine the effect of salidroside on the migration ability of 97H cells.Transwell assay was used to determine the effect of salidroside on the invasion ability of 97H cells.Inverted microscope was used to observe the effect of salidroside on the morphology of 97H cells.Transmission electron microscope was employed to observe the effect of salidroside on mitochondria in 97H cells.Flow cytometry was used to analyze the effect of salidroside on apoptosis and cycle distribution of 97H cells.q-PCR was used to determine the effect of salidroside on related apoptosis genes in 97H cells.Western blot was used to determine the effect of salidroside on related migration,invasion and apoptosis proteins in 97H cells.Results Compared to blank group,salidroside treatment inhibited the proliferation,migration and invasion of 97H cells,and induced apoptosis of 97H cells.Salidroside could up-regulate the relative expression of Caspase-3(P<0.05).Salidroside could up-regulate the levels of E-cad,Bax,Caspase-3 and Caspase-9 proteins(P<0.05),and down-regulate the levels of N-cad,Girdin and Bcl-2 proteins(P<0.05).Conclusions Salidroside has inhibitory effects on the proliferation,migration and invasion of 97H cells,and induces apoptosis of 97H cells through mitochondrial pathway.