文献类型：期刊文献

英文题名：Cool Temperature Enhances Growth, Ferulic Acid and Flavonoid Biosynthesis While Inhibiting Polysaccharide Biosynthesis in Angelica sinensis

作者：Dong, Han[1];Li, Meiling[2];Jin, Ling[1];Xie, Xiaorong[1];Li, Mengfei[2];Wei, Jianhe[3]

第一作者：Dong, Han

通信作者：Xie, XR[1];Li, MF[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730030, Peoples R China;[2]Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China;[3]Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730030, Peoples R China;[2]corresponding author), Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2022

卷号：27

期号：1

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000752642700001)】；

基金：This research was financially supported by funds from the National Natural Science Foundation of China (32160083); China Agriculture Research System of MOF and MARA (CARS-21); State Key Laboratory of Aridland Crop Science Gansu Agricultural University (GSCS-2021-Z03); Key talent projects of Gansu Province, China (2020RCXM103); and Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003).

语种：英文

外文关键词：Angelica sinensis; cool temperature; growth; ferulic acid biosynthesis; flavonoid biosynthesis; gene expression

摘要：Angelica sinensis, a perennial herb that produces ferulic acid and phthalides for the treatment of cardio-cerebrovascular diseases, prefers growing at an altitude of 1800-3000 m. Geographical models have predicted that high altitude, cool temperature and sunshade play determining roles in geo-authentic formation. Although the roles of altitude and light in yield and quality have been investigated, the role of temperature in regulating growth, metabolites biosynthesis and gene expression is still unclear. In this study, growth characteristics, metabolites contents and related genes expression were investigated by exposing A. sinensis to cooler (15 degrees C) and normal temperatures (22 degrees C). The results showed that plant biomass, the contents of ferulic acid and flavonoids and the expression levels of genes related to the biosynthesis of ferulic acid (PAL1, 4CLL4, 4CLL9, C3H, HCT, CCOAMT and CCR) and flavonoids (CHS and CHI) were enhanced at 15 degrees C compared to 22 degrees C. The contents of ligustilide and volatile oils exhibited slight increases, while polysaccharide contents decreased in response to cooler temperature. Based on gene expression levels, ferulic acid biosynthesis probably depends on the CCOAMT pathway and not the COMT pathway. It can be concluded that cool temperature enhances plant growth, ferulic acid and flavonoid accumulation but inhibits polysaccharide biosynthesis in A. sinensis. These findings authenticate that cool temperature plays a determining role in the formation of geo-authentic and also provide a strong foundation for regulating metabolites production of A. sinensis.