文献类型：期刊文献

英文题名：A novel "Turn-On" fluorescent probe for F detection in aqueous solution and its application in live-cell imaging

作者：Xu, Jian[1,2,3];Sun, Shaobo[4];Li, Qian[1,2];Yue, Ying[1,2];Li, Yingdong[4];Shao, Shijun[1,2]

第一作者：Xu, Jian

通信作者：Shao, SJ[1]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100039, Peoples R China;[4]Gansu Univ Chinese Med, Inst Integrated Tradit & Western Med, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.

年份：2014

卷号：849

起止页码：36

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20143600024673);Scopus(收录号：2-s2.0-84908067465);WOS:【SCI-EXPANDED(收录号:WOS:000343095900006)】；

基金：This work was supported by the National Natural Science Foundation of China (20972170 and 21275150), the Funds for Distinguished Young Scientists of Gansu (1210RJDA013) and the Natural Science Foundation of Gansu province (1107RJYA069).

语种：英文

外文关键词："Turn-On" fluorescent probe; Fluoride ions sensing; DFT calculation; Live-cell imaging; A549 and ATII cells

摘要：A novel probe incorporating quaternized 4-pyridinium group into a BODIPY molecule was synthesized and studied for the selective detection of fluoride ions (F) in aqueous solution. The design was based on a fluoride-specific desilylation reaction and the "Turn-On" fluorescent response of probe 1 to F was ascribed to the inhibition of photoinduced electron transfer (PET) process. The probe displayed many desired properties such as high specificity, appreciable solubility, desirable response time and low toxicity to mammalian cells. There was a good linearity between the fluorescence intensity and the concentrations of F in the range of 0.1-1 mM with a detection limit of 0.02 mM. The sensing mechanism was confirmed by the NMR, electrospray ionization mass spectrum, optical spectroscopy and the mechanism of "Turn-On" fluorescent response was also determinated by a density functional theory (DFT) calculation using Gaussian 03 program. Moreover, the probe was successfully applied for the fluorescence imaging of F in human epithelial lung cancer (A549) cells and alveolar type II (ATII) cells under physiological conditions. (C) 2014 Elsevier B.V. All rights reserved.