文献类型：期刊文献

英文题名：Identification of key gene networks controlling vernalization development characteristics of Isatis indigotica by full-length transcriptomes and gene expression profiles

作者：Wang, Pan[1];Liu, Dong[1];Yang, Fu-Hong[1,2];Ge, Hui[1];Zhao, Xin[1];Chen, Hong-Gang[1];Du, Tao[1]

第一作者：王佩;王萍

通信作者：Du, T[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]Pingliang Acad Agr Sci, Pingliang 744000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2021

卷号：27

期号：12

起止页码：2679

外文期刊名：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000734011600001)】；

基金：This research was funded by China Agriculture Research System of MOF and MARA (CARS-21) and Study on Selection and Breeding Methods and Planting Quality Control Techniques of Excellent Varieties of Isatis indigotica (201507002-1-07).

语种：英文

外文关键词：Isatis indigotica Fort; Vernalization; Nanopore sequencing; Transcript; WGCNA

摘要：Isatis indigotica Fort., as a common Chinese medicinal raw material, will lose its medicinal value if it blooms early, so it is highly valuable to clarify the induction mechanism of the vernalization of I. indigotica at low temperature. In this study, the concentrations of soluble sugar, proline, glutathione and zeatin in two germplasms of I. indigotica with different degrees of low temperature tolerance (Y1 and Y2) were determined at 10 days, 20 days and 30 days of low-temperature treatment, and the full-length transcriptome of 24 samples was sequenced by Nanopore sequencing with Oxford Nanopore Technologies (ONT). After that, the data of transcripts involved in the vernalization of I. indigotica at low temperature were obtained, and these transcripts were identified using weighted gene co-expression network analysis (WGCNA). The results revealed the massive accumulation of soluble sugar and proline in Y1 and Y2 after low temperature induction. A total of 18,385 new transcripts, 6168 transcription factors and 470 lncRNAs were obtained. Differential expression analysis showed that gibberellin, flavonoids, fatty acids and some processes related to low temperature response were significantly enriched. Eight key transcripts were identified by WGCNA, among which ONT.14640.1, ONT.9119.1, ONT.13080.2 and ONT.16007.1 encodes a flavonoid transporter, 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), growth factor gene and L-aspartate oxidase in plants, respectively. It indicated that secondary metabolites such as hormones and flavonoids play an important role in the vernalization of I. indigotica. qRT-PCR proved the reliability of transcriptome results. These results provide important insights on the low-temperature vernalization of I. indigotica, and provide a research basis for analyzing the vernalization mechanism of I. indigotica.