文献类型：期刊文献

中文题名：高乌头三氯甲烷部分对肝癌细胞的作用

英文题名：Effects of chloroform extract of chloroform extract of Aconitum sinomontanum Nakai on hepatocellular carcinoma cells

作者：魏家艳[1,2,3];贾春艳[1,2,3];张国玉[1,2,3];熊继东[1,2,3];仲楠[1,2,3];强宇靖[1,2,3];杨锡仓[4];李芸[1,2,3,4]

第一作者：魏家艳

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃中医药大学陇药产业创新研究院,甘肃兰州730000;[3]甘肃中医药大学西北中藏药省部共建协同创新中心,甘肃兰州730000;[4]甘肃中医药大学附属医院杨锡仓名老中医药专家传承工作室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2024

卷号：40

期号：24

起止页码：3568

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金资助项目(82360773);甘肃省重点研发计划基金资助项目(20YF2FA019);甘肃中医药大学研究生创新创业基金资助项目(2022CX84)。

语种：中文

中文关键词：高乌头三氯甲烷部分;肝癌;HepG2细胞

外文关键词：Aconitum sinomontanum Nakai;liver cancer;HepG2 cells

摘要：目的探讨高乌头三氯甲烷部分的抗肝癌活性。方法细胞实验:将HepG2细胞分为空白组(0.9%NaCl)和低、中、高实验组(2、4、8 mg·mL^(-1)高乌头三氯甲烷部分),用噻唑蓝(MTT)法检测24、48、72 h细胞增殖活力情况。动物实验:将接种了H22细胞的BALB/C小鼠分为模型组(0.9%NaCl)、顺铂组(2 mg·kg^(-1)顺铂)、氢溴酸高乌甲素组(4 mg·kg^(-1)氢溴酸高乌甲素)和高剂量组(8 mg·kg^(-1)高乌头三氯甲烷部分),另取BALB/C小鼠为对照组(0.9%NaCl),连续给药14 d。检测高乌头三氯甲烷部分的抑瘤率,用酶联免疫吸附试验(ELISA)法检测各组小鼠血清中炎症、肝癌和肝功能相关因子指标,用免疫组化法检测肿瘤组织凋亡蛋白。结果高乌头三氯甲烷部分对HepG2细胞给药24、48和72 h的半数抑制浓度(IC50)分别为5.71、4.37和2.12 mg·mL^(-1)。对照组、模型组、顺铂组、氢溴酸高乌甲素组和高剂量组小鼠血清中甲胎蛋白(AFP)的表达水平分别为8.84±0.35、12.04±0.76、10.14±1.01、9.53±0.79和9.33±1.06。顺铂组、氢溴酸高乌甲素组和高剂量组抑瘤率分别为48.40%、50.71%和52.58%。模型组、顺铂组、氢溴酸高乌甲素组和高剂量组的B细胞淋巴瘤/白血病-2(Bcl-2)表达水平分别为101.09±7.15、65.92±6.11、67.12±7.88和62.60±10.75,促凋亡蛋白Bcl-2家族相关X蛋白(Bax)相对表达水平分别为48.57±15.50、89.09±8.54、60.40±3.24和108.79±3.17。顺铂组、氢溴酸高乌甲素组和高剂量组的上述指标与模型组相比,在统计学上差异均有统计学意义(P<0.01,P<0.05)。结论高乌头三氯甲烷部分具有显著的抗肝癌活性,抑制肝癌细胞的增殖,诱导其凋亡,从而发挥抗肝癌活性。
Objective To investigate Aconitum sinomontanum Nakai has anti-hepatoma activity.Methods Cell experiment:HepG2 cells were divided into blank group(0.9%NaCl)and experimental-L,-M,-H groups(2,4,8 mg·mL^(-1)Aconitum sinomontanum Nakai).The24,48,72 h cell proliferation activity was detected by methyl thiazolyl tetrazolium(MTT)method.Animal experiments:BA LB/C mice inoculated with H22 cells were divided into model group(0.9%NaCl),cisplatin group(2 mg·kg^(-1)cisplatin),lappaconitine hydrobromide group(4 mg·kg^(-1)lappaconitine hydrobromide)and high-dose group(8 mg·kg^(-1)Aconitum sinomontanum Nakai).BA LB/C mice were selected as control group(0.9%NaCl).After 14 days of continuous administration,the tumor inhibition rate of Aconitum sinomontanum Nakai was detected.The indexes of inflammation,liver cancer and liver function related factors in serum of mice in each group were detected by enzyme-linked immunosorbent assay(ELISA).The apoptosis protein of tumor tissue was detected by immunohistochemistry.Results The median inhibitory concentration(IC_(50))of trichloromethane in HepG2cells for 24,48 and 72 h were 5.71,4.37 and 2.12 mg·mL^(-1),respectively.The expression levels of alphafetoprotein(AFP)in serum were 8.84±0.35,12.04±0.76,10.14±1.01,9.53±0.79 and 9.33±1.06 in control group,model group,cisplatin group,lappaconitine hydrobromide group and high-dose group,respectively.The tumor inhibition rates of cisplatin group,lappaconitine hydrobromide group and high-dose group were 48.40%,50.71%and 52.58%,respectively.The expression levels of B-cell lymphoma-2(Bel-2)in model group,cisplatin group,lappaconitine hydrobromide group and high-dose group were 101.09±7.15,65.92±6.11,67.12±7.88 and 62.60±10.75,respectively;the expression levels of pro-apoptotic protein Bcl-2 associated X protein(Bax)were 48.57±15.50,89.09±8.54,60.40±3.24 and 108.79±3.17,respectively.Compared with the model group,the above indexes in cisplatin group,hyperaconitine hydrobromide group and high-dose group had statistical significance(P<0.01,P<0.05).Conclusion Aconitum sinomontanum Nakai has significant anti-liver cancer activity,inhibits the proliferation of hepatoma cells,induces apoptosis,and thus exerts anti-hepatocarcinoma activity.