文献类型：期刊文献

中文题名：当归挥发油对人肺腺癌SPC-A-1细胞增殖及凋亡的影响

英文题名：Effect of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell SPC-A-1

作者：张艳霞[1];张延英[1];张正顺[2];安方玉[1];陈绍仪[3];周佳玮[1];孔学礼[1]

第一作者：张艳霞

机构：[1]甘肃中医药大学/甘肃省中药药理与毒理学重点实验室/甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000;[2]甘肃省中医院白银分院,白银730900;[3]白银市第二人民医院,白银730900

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2016

卷号：32

期号：6

起止页码：102

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：白银市科技计划项目(2013-2-76Y);甘肃中医学院中青年科研基金项目(2305016601)

语种：中文

中文关键词：当归挥发油;人肺腺癌SPC-A-1细胞;细胞凋亡

外文关键词：volatile oil of the Angelica（当归挥发油） ; human lung adenocarcinoma cell SPC-A-1 ; apoptosis

摘要：目的：探讨当归挥发油对人肺腺癌SPC-A-1细胞增殖及凋亡的作用。方法：MTT比色法检测当归挥发油对SPC-A-1细胞的细胞毒作用;流式细胞术检测SPC-A-1细胞凋亡及周期;吖啶橙/溴化乙啶（AO/BE）双荧光染色法观察SPC-A-1细胞凋亡。结果：在6.25-400μg/ml的浓度范围内,当归挥发油对SPC-A-1细胞的增殖具有浓度依赖性抑制作用,其对SPC-A-1细胞的IC50为112.38μg/ml。流式细胞术检测结果表明,当归挥发油可诱导SPC-A-1细胞凋亡、坏死,且细胞凋亡、坏死率随药物浓度增高而升高。AO/BE双荧光染色法结果显示,用药组随着药物浓度的升高,细胞结构固缩加重,凋亡、坏死细胞增多。结论：当归挥发油可诱导SPC-A-1细胞凋亡。
Objective： To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell SPC-A-I. Methods： The cytotoxic effect of human lung adenocarcinoma cell SPC-A-1 was investigated by MTT colorimetric assay. The cell apoptosis and cell cycle were analyzed by flow cytometry. The cell apoptosis was observed by AO/BE fluorescent staining method. Results： The SPC- A-1 cell was inhibited by volatile oil of the angelica in a concentration-dependent manner, the IC50 was 112.38 μg/ml. The result of flow cy-tometry indicate that, volatile oil of the angelica could induced apoptosis and necrosis of SPC-A-1 cell, and the rate of apoptosis and necrosis all rose with the raise of drug concentration. The results of AO/BE fluorescent staining method displayed that, the rates of apoptosis and nec- rosis all rose in drug groups. The result of flow eytometry indicated that, after using volatile oil of the angelica, the SPC-A-1 cell cycle was blocked in G2. Conclusion ： Volatile oil of the angelica had depressant effect on the generation of SPC-A-1 cell, the mechanism is probably that it can induce apoptosis of SPC-A-1 cell.