文献类型：期刊文献

中文题名：乳康胶囊对乳腺增生大鼠激素及细胞凋亡水平的影响

英文题名：Rukang Capsules Has Effect on Levels of Hormones and Apoptosis in Rats with Hyperplasia of Mammary Glands

作者：叶延程[1];丁高恒[2];宋鹏[3];杨宁[2];高青[2];张娜[2];赵良存[1,2]

第一作者：叶延程

机构：[1]甘肃省武威肿瘤医院,甘肃武威734500;[2]甘肃中医药大学公共卫生学院,甘肃兰州730000;[3]甘肃中医药大学附属第二医院,甘肃兰州730000

第一机构：甘肃省武威肿瘤医院,甘肃武威734500

年份：2019

卷号：0

期号：6

起止页码：8

中文期刊名：新中医

外文期刊名：New Chinese Medicine

基金：甘肃省中医药防治慢性疾病重点实验室开放基金项目(GSMBKY2015-11,GSMBKY2015-10);兰州市城关区科技局基金项目(2014-4-5)

语种：中文

中文关键词：乳康胶囊;乳腺增生症(HMG);雌激素;细胞凋亡;动物实验;大鼠

外文关键词：Rukang capsules;Hyperplasia of mammary gland(HMG);Estrogen;Apoptosis;Animal experiment;Rats

摘要：目的:观察乳康胶囊对乳腺增生症(Hyperplasia of mammary gland,HMG)大鼠血清激素、细胞凋亡的影响及治疗作用。方法:采用注射苯甲酸雌二醇、黄体酮建立大鼠HMG模型,将造模成功的大鼠随机分为6组,即正常组、模型组、阳性药组和乳康胶囊低、中、高剂量组,给药30天后,测量各组大鼠同侧第2对乳头的高度及直径;采用酶联免疫吸附(ELISA)法检测血清雌二醇(Estradiol 2,E2)、孕酮(Progesterone,P)和促乳素(Prolactin,PRL)的含量;用免疫组化法检测各组大鼠乳腺组织B淋巴细胞瘤相关蛋白质(B-cell lymphoma-associated X protein,Bax)、B淋巴细胞瘤-2基因(B-cell lymphoma 2,Bcl-2)表达。结果:与正常组比较,模型组大鼠乳头直径、乳头高度增加(P <0.05);与模型组比较,各给药组乳房直径、乳头高度降低(P <0.05);血清E2、PRL含量降低,P含量升高(P <0.05);乳康胶囊高剂量组乳腺组织Bax表达升高,Bcl-2表达降低(P <0.05);与阳性药组比较,乳康胶囊高剂量组乳房直径减小(P <0.05),乳康胶囊各剂量组E2、PRL、P含量及Bax、Bcl-2表达差异无统计学意义。结论:乳康胶囊能减轻HMG发展,其作用与降低大鼠血清E2、PRL以及升高P含量有关,可能与通过调节Bax、Bcl-2途径促进细胞凋亡有关。
Objective:To observe the effect of Rukang capsules on the hormones of serum and apoptosis in rats with hyperplasia of mammary gland(HMG)and its curative effect.Methods:The rat models with HMG were established by injecting estradiol benzoate and progesterone.Rats successfully modelled were randomly divided into six groups,namely the normal group,the model group,the positive medicine group and the Rukang capsules groups of low-dose,medium-dose and high-dose.After 30-day administration of medicine,the heights and diameters of the ipsilateral second pair of nipples of rats in each group were measured;the contents of the estradiol 2(E2),the progesterone(P)and the prolactin(PRL)in serum were detected through Enzyme-Linked Immunosorbent Assay(ELISA);the expressions of B-cell lymphoma-associated X protein(Bax)and B-cell lymphoma 2(Bcl-2)in mammary-glands tissues of rats in each group were detected through the immunohistochemistry.Results:Compared with those in the normal group,the height and diameter of the nipples of rats in the model group were increased(P<0.05);compared with those in the model group,the height and diameter of the nipples in each group after administration of medicine were decreased(P<0.05);the contents of E2 and PRL in serum were decreased while the content of P was increased(P<0.05);the expression of Bax in the mammary-glands tissues in the Rukang capsules group of high-dose was increased,while the expression of Bcl-2 was decreased(P<0.05);compared with those in the positive medicine group,the diameter of the nipples of rats in the Rukang capsules group of high-dose was decreased(P<0.05),and compared with the contents of E2,PRL and P as well as the expressions of Bax and Bcl-2 in the Rukang capsules groups of each dose respectively,there was no significance in the difference.Conclusion:Rukang capsules can alleviate the advancement of HMG,whose effect is related to the decrease of the contents of E2 and PRL as well as the increase of the content of P in serum in rats and may be related to the promotion of apoptosis through regulating Bax and Bcl-2.