文献类型：期刊文献

中文题名：黄芪注射液干预人宫颈永生化上皮细胞体外实验模型的细胞凋亡变化

英文题名：Effects of astragalus injection on human immortalized cervical epithelial cell apoptosis in vitro

作者：吕玲[1];肖晨光[2];刘青[1];张立[3];李能莲[3];舍雅莉[3]

第一作者：吕玲

机构：[1]甘肃省妇幼保健院,甘肃省兰州市730050;[2]中核兰州铀浓缩有限公司职工医院,甘肃省兰州市730065;[3]甘肃中医学院,甘肃省兰州市730000

第一机构：甘肃省妇幼保健院,甘肃省兰州市730050

年份：2016

卷号：20

期号：5

起止页码：743

中文期刊名：中国组织工程研究

外文期刊名：Chinese Journal of Tissue Engineering Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；

基金：甘肃省中医药管理局课题(GZK-2013-56)~~

语种：中文

中文关键词：黄芪;上皮细胞;细胞凋亡;组织工程;实验动物;细胞损伤与修复模型;黄芪注射液;宫颈永生化

外文关键词：Astragalus membranaceus; Epithelial Cells; Apoptosis; Tissue Engineering

摘要：背景:宫颈永生化上皮细胞H8在致癌因素诱导下可以发生癌变,当有协同因子共同作用时就会导致宫颈癌的发生。但临床上对于癌前病变的女性尚缺乏有效的干预治疗,此项治疗临床为空白。目的:分析黄芪注射液对人宫颈永生化上皮细胞(H8细胞)凋亡的作用及机制。方法:实验分2组,黄芪注射液药物组和空白对照组。1ELISA法检测黄芪注射液作用后人宫颈永生化上皮细胞H8凋亡的DNA片断。2酶标仪分析黄芪注射液作用后人宫颈永生化上皮细胞H8中凋亡酶caspase-3、caspase-9酶活性的变化。3Western blot检测黄芪注射液作用后,人宫颈永生化上皮细胞H8中caspase-3、caspase-9、PARP蛋白表达的变化。结果与结论:1ELISA法检测,20 g/L黄芪注射液分别作用0,6,12,24 h后,人宫颈永生化上皮细胞H8DNA片段随着药物作用时间的延长逐渐增多,且呈时间依赖性(P<0.05)。2酶标仪检测,20 g/L黄芪注射液作用0,6,12,24 h后,人宫颈永生化上皮细胞H8 caspase-3和caspase-9的活性增高,且呈时间依赖性(P<0.05)。320 g/L黄芪注射液分别作用0,6,12和24 h后,人宫颈永生化上皮细胞H8中cleaved caspase-3、cleaved caspase-9表达逐渐升高,差异有显著性意义(P<0.05);Cleaved PARP蛋白的表达逐渐下降,差异有显著性意义(P<0.05)。结果说明,黄芪注射液对人宫颈永生化上皮细胞H8具有较显著的诱导凋亡作用,其机制可能与上调caspase-3和caspase-9蛋白表达有关。
BACKGROUND： Immortalized cervical epithelial cells H8 can become cancerous under the induction of carcinogenic agent, and may cause cervical cancer when there is a cofactor interaction. However, there is still a lack of effective intervention for female patients with precancerous lesions, and this treatment is blank in the clinic. OBJECTIVE： To explore the effects and mechanism of astragalus injection on apoptosis of human immortalizedcervical epithelial cells H8. METHODS： This study contained two groups： astragalus drug group and the blank control group.（1） Enzyme linked immunosorbent assay（ELISA） was used to detect DNA fragments of apoptotic H8 after astragalus injection.（2） Enzyme-labeling instrument was used to analyze the changes in caspase-3 and caspase-9 activities a fter astragalus injection.（3） Western blot assay was used to detect the protein expression changes of caspase-3, caspase-9 and PARP in H8 cells after astragalus injection. RESULTS AND CONCLUSION：（1） ELISA results showed that at 0, 6, 12 and 24 hours after 20 g/L astragalus injection, DNA fragments were gradually increased with time prolonged in a time-dependent effect（P〈0.05）.（2） Enzyme-labeling instrument demonstrated that at 0, 6, 12 and 24 hours after 20 g/L astragalus injection, caspase-3 and caspase-9 activities increased in a time-dependent manner（P〈0.05）.（3） At 0, 6, 12 and 24 hours after 20 g/L astragalus injection, the expression of cleaved caspase-3 and cleaved caspase-9 were gradually increased in H8 cells（P〈0.05）. Cleaved PARP protein expression was gradually decreased（P〈0.05）. These findings indicate that astragalus injection could obviously induce H8 apoptosis, which may be associated with the upregulated protein expression of caspase-3 and caspase-9.