文献类型：期刊文献

英文题名：Berberine Damages the Cell Surface of Methicillin-Resistant Staphylococcus aureus

作者：Zhang, Xiujuan[1,2];Sun, Xiaoying[1];Wu, Jiaxin[1];Wu, Yue[3];Wang, Yali[2];Hu, Xiaoqing[1,2,4];Wang, Xiaoyuan[1,4]

第一作者：张秀娟;Zhang, Xiujuan

通信作者：Hu, XQ[1];Wang, YL[2];Hu, XQ[2];Hu, XQ[3]

机构：[1]Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Key Lab Qual & Stand Res Tradit Chinese Med Gansu, Lanzhou, Peoples R China;[3]Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Peoples R China;[4]Jiangnan Univ, Int Joint Lab Food Safety, Wuxi, Jiangsu, Peoples R China

第一机构：Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

通信机构：[1]corresponding author), Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China;[2]corresponding author), Gansu Univ Tradit Chinese Med, Key Lab Qual & Stand Res Tradit Chinese Med Gansu, Lanzhou, Peoples R China;[3]corresponding author), Jiangnan Univ, Int Joint Lab Food Safety, Wuxi, Jiangsu, Peoples R China.|[10735]甘肃中医药大学;

年份：2020

卷号：11

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85084454527);WOS:【SCI-EXPANDED(收录号:WOS:000533383300001)】；

基金：This research was supported by the Open Project Fund of Key Laboratory of Quality and Standard Research of Traditional Chinese Medicine in Gansu Province (ZYZL18-003), National Natural Science Foundation of China (31201290) and National First-Class Discipline, and Program of Light Industry Technology and Engineering (LITE2018-10).

语种：英文

外文关键词：berberine; cell surface; Staphylococcus aureus; damage; integrity; fatty acids

摘要：Methicillin-resistant Staphylococcus aureus (MRSA) is currently regarded as one of the most important drug-resistant pathogens causing nosocomial and community-acquired infections. Although berberine (BER) has shown anti-MRSA activity, the underlying mechanism is still unclear. In this study, the damage caused by BER on the cell surface of MRSA was systematically investigated by performing BER susceptibility test, determining K+ and alkaline phosphatase (ALP) release, detecting morphological alterations using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and ascertaining lipid profiles. The results showed that the minimum inhibitory concentration (MIC) of BER against MRSA252 was 128 mu g/ml. Under the sub-MIC doses of BER, cell membrane permeability gradually increased in a dose-dependent manner, and 1 x MIC led to 43.8% higher K+ leakage and fourfold higher ALP secretion. The injuries on MRSA cell surface were further verified by SEM and TEM, and some cells displayed a doughnut-shaped structure. BER significantly altered the fatty acid species contents, including saturated fatty acids (C-14(:)0, C-15(:)0, C-16(:)0, C-18(:)0, and C-20(:)0), and unsaturated fatty acids (C-20(:)4, C-20(:)1, and C-18(:)1), indicating that BER compromised cell membrane integrity via lipid fluctuation. Thus, the findings of this study could help to unravel the molecular mechanism of BER against MRSA.