文献类型：期刊文献

英文题名：Simultaneous Separation and Identification of the Diastereoisomers of Epipodophyllotoxin and its Esters by Liquid Chromatography-Diode Array Detection and -Electrospray Ionization Mass Spectrometry

作者：Zhao, Lei[2];Liu, Lina[2];Fan, Pengcheng[3];Zhang, Jiaqiang[1];Wu, Xiaoyu[3];Yu, Xiaohui[2];Tian, Xuan[1]

第一作者：赵磊

通信作者：Tian, X[1]

机构：[1]Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China;[2]Gansu Coll Tradit Chinese Med, Coll Gansu Prov, Key Lab Chem & Qual Tradit Chinese Med, Lanzhou 730000, Peoples R China;[3]Gen Hosp Lanzhou, Dept Pharm, Lanzhou 730050, Peoples R China

第一机构：Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, State Key Lab Appl Organ Chem, 222 Tianshui Rd, Lanzhou 730000, Peoples R China.

年份：2011

卷号：7

期号：3

起止页码：202

外文期刊名：CURRENT PHARMACEUTICAL ANALYSIS

收录：;Scopus(收录号：2-s2.0-79960629182);WOS:【SCI-EXPANDED(收录号:WOS:000294418800008)】；

基金：This research was supported in part by the Natural Science Foundation of Gansu Province (3ZS061-A25-O24). Our hearty thanks go to Professor Cui-Rong Sun and Ms. JuanJuan Chen (Zhejiang University) for performing the HPLC-ESI/MS analysis.

语种：英文

外文关键词：Diastereoisomers separation; Epipodophyllotoxin; High performance liquid chromatography-electro spray tandem; mass spectrometry; Relative abundance

摘要：Epipodophyllotoxin and its derivatives are important derivatives of podophyllotoxin which are used in cancer chemotherapy. The conditions for the separation and identification of a mixture of seven diastereoisomer pairs at the C-2 position of epipodophyllotoxin and its esters were optimized using both high-performance liquid chromatography-diode array detection (HPLC-DAD) and high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS). The separation was carried out on a BDS Hypersil C(18) column with methanol-acetonitrilewater as the mobile phase in a gradient elution program. In the HPLC-ESI/MSspectra, the [M+Na](+) ions of all seven pairs were observed. The ion ratios of the relatively abundant ions of [M-ROH+H](broken vertical bar) (ion 397 or 383) to [M+Na](broken vertical bar) , [A+ H](broken vertical bar) ( ion 313) to [M-ROH+H](+), and [A+H-OCH(3)](+) ( ion 282) to [M-ROH+H](+) of each diastereoisomer pair of the lignans specifically exhibited a stereochemical effect. Thus, using identical chromatographic conditions, the combination of DAD and MS permits the separation and identification of seven diastereoisomers of epipodophyllotoxin at the C-2 position. The results of this work may serve as an effective tool for the separation and detection of other epipodophyllotoxin derivatives and their analogues.