文献类型：期刊文献

英文题名：Agaricus blazei extract (FA-2-b-β) induces apoptosis in chronic myeloid leukemia cells

作者：Sun, Yanqing[1,2];Cheng, Mingxia[1,2];Dong, Li[2];Yang, Kehu[3,4];Ma, Zhiyuan[5];Yu, Shangrui[6];Yan, Peijing[4];Bai, Kuntian[2];Zhu, Xiaolong[1];Zhang, Qike[1]

第一作者：Sun, Yanqing;孙永强

通信作者：Sun, YQ[1]

机构：[1]Gansu Prov Hosp, Dept Hematol, Lanzhou, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Dept Clin Med, Lanzhou, Peoples R China;[3]Lanzhou Univ, Hosp 1, Sch Basic Med Sci, Evidence Based Med Ctr, Lanzhou, Peoples R China;[4]Lanzhou Univ, Hosp 1, Inst Clin Res & Evidence Based Med, Lanzhou, Peoples R China;[5]Lanzhou Univ, Hosp 1, Dept Gastroenterol, Lanzhou, Peoples R China;[6]Lanzhou Univ, Hosp 2, Dept Gastroenterol, Lanzhou 730000, Gansu, Peoples R China

第一机构：Gansu Prov Hosp, Dept Hematol, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Hematol, 204 Gansu Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2020

卷号：20

期号：5

外文期刊名：ONCOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000590173300148)】；

基金：The present study was funded by Gansu Provincial Department of Science and Technology (grant no. GZK-2013-8) and the Natural Science Foundation of China (grant no. 8156140621).

语种：英文

外文关键词：CML; AbM; Wnt; beta-catenin signaling pathway; apoptosis cycle

摘要：Agaricus blazei Murill (AbM) is a mushroom belonging to the Basidiomycetes family, which is believed to have antitumor and antioxidative activities. Proteoglycans and ergosterol are considered the key compounds of AbM for antitumor properties and so are used in complementary and alternative medicine as an anticancer drug. AbM is used to avoid serious side effects that would inevitably affect patients. Currently, the efficacy of AbM against chronic myeloid leukemia (CML) has not been established. The present study aimed to investigate the antitumor activities of the acidic RNA protein complex, FA-2-b-beta, extracted from wild edible AbM. The CML K562 cells or primary CML bone marrow (BM) cells were treated with FA-2-b-beta at different concentrations and time points. CML cell line proliferation and apoptosis were determined using the CCK-8 assay or Annexin V/propidium iodide (PI) labeling, RT-qPCR and western blotting was performed to determine the involvement of the Wnt/beta -catenin-associated apoptotic pathway. The results of the present study demonstrated that FA-2-b-beta has a high anti-proliferative potency and strong pro-apoptotic effects. Thus, daily intake of mushrooms containing FA-2-b-beta may be an adequate source as an alternative medicine in the management of CML, and may provide useful information for the development of a novel therapeutic target in this area.