文献类型：期刊文献

中文题名：藏药熏倒牛提取物的抑菌杀虫活性研究

英文题名：Study on the Anti-bacterial and Anthelminthic effects of Biebersteinia heterostemon Maxim

作者：张艳霞[1,2];景明[1,2];王晶晶[1];李炀[1];张银川[3]

第一作者：张艳霞

机构：[1]甘肃中医学院;[2]甘肃省中药药理学与毒理学重点实验室,兰州730000;[3]定西市妇幼保健院,定西742000

第一机构：甘肃中医药大学

年份：2014

卷号：26

期号：12

起止页码：2054

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：兰州市社会发展科技计划(2012-1-57)

语种：中文

中文关键词：熏倒牛;活性部位;抑菌杀虫

外文关键词：Biebersteinia heterostemon Maxim; active fraction; anti-bacterial and anthelminthic

摘要：为了探讨藏药熏倒牛乙醇提取物不同极性溶剂萃取部位的抑菌杀虫活性,将熏倒牛乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取,所得各萃取部位及水余液部位通过抑菌圈法测定抑菌活性部位,对活性部位采用二倍稀释法测定最低抑菌浓度,采用改良任氏法测定杀灭滴虫活性部位及最低有效杀虫浓度。结果表明熏倒牛乙醇提取物的乙酸乙酯部位对金黄色葡萄球菌、铜绿假单胞菌和阴沟肠杆菌有强抑制作用,最低抑菌浓度为0.125 mg/m L,对变形杆菌、粪肠球菌和白色念珠菌的抑制作用也比较强,最低抑菌浓度为0.25 mg/m L。石油醚部位和乙酸乙酯部位对阴道滴虫有明显抑制作用,最低有效杀虫浓度分别为0.5、0.25 mg/m L。说明熏倒牛乙醇提取物的乙酸乙酯萃取部位有较强的抑菌杀虫作用,是熏倒牛的抑菌杀虫活性部位。
The aim of this study was to investigate the anti-bacterial and anthelminthic effects of different fractions of ethanol extract of Biebersteinia heterostemon Maxim. The B. heterostemon sample was firstly extracted by ethanol. The ethanol extract was then fractionated using petroleum ether,ethyl acetate and n-butanol sequentially. The anti-bacterial effects of different extracts of B. heterostemon were tested using the bacteriostatic cycle method. The minimal inhibitory concentrations（ MICs） were determined by double dilution method. The modified Ren＇s method was used to determine the active fraction with anthelminthic effect and its respective minimal effective concentration（ MEC）. The results showed that the ethyl acetate fraction of B. heterostemon ethanol extract had strong inhibitory effect on Staphylococcus aureus,Pseudomonas aeruginosa and Enterobacter cloacae,with MIC of 0. 125 mg / m L. Its inhibitory effect on Bacillus proteus,Enterococcus faecalis and Blastomyces albicans were also strong with MIC of 0. 25 mg / m L. Both petroleum ether fraction and ethyl acetate fraction had obviously inhibitory effect on Trichomonas vaginalis with MICs of 0. 5 mg / m L and 0. 25 mg /m L,respectively. Based on these experimental results,it was concluded that the ethyl acetate fraction of B. heterostemon ethanol extract had strong anti-bacterial and anthelminthic effects.