文献类型：期刊文献

英文题名：Integrative multi-omics reveals the protective function and molecular weight components of Astragalus polysaccharide against chemotherapeutic intestinal mucositis in Drosophila and mice

作者：Qin, Yujie[1];Wang, Yan[1];Wu, Jinhan[2,3];Wang, Xiaoqian[2,3];Fu, Jinyu[2,3];Wang, Junlong[4];Lin, Xingyao[5];Xiu, Minghui[1,5];Liu, Yongqi[2,3,5];He, Jiangzheng[5,6,7]

第一作者：Qin, Yujie

通信作者：Xiu, MH[1];Liu, YQ[1];He, JZ[1]

机构：[1]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Prevent & Treatment Tradit Chinese Med Res Gansu C, Lanzhou 730000, Peoples R China;[4]Northwest Normal Univ, Coll Life Sci, Lanzhou 730000, Peoples R China;[5]Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China;[6]Gansu Univ Chinese Med, Res & Expt Ctr, Lanzhou 730000, Peoples R China;[7]Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gansu, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Minist Educ, Key Lab Dunhuang Med, Lanzhou 730000, Peoples R China.

年份：2025

卷号：316

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20252218502589);Scopus(收录号：2-s2.0-105006717922);WOS:【SCI-EXPANDED(收录号:WOS:001504604600005)】；

基金：This work was supported by the Gansu Higher Education Industry Support Plan Project (No. 2025cYzc-47) , National Natural Science Foundation of China (No. 82360896) , Postdoctoral Research Founda-tion of China (No. 2023MD734146) , Foundation from NHC Key Labo-ratory of Diagnosis and Therapy of Gastrointestinal Tumor (No. NHCDP2022006) .

语种：英文

外文关键词：Astragalus polysaccharide; Chemotherapeutic intestinal mucositis; Molecular weight components; FoxO signaling related oxidative stress; Innate immune response; Intestinal flora imbalance

摘要：Astragalus polysaccharide (APS) is the crucial active ingredient of Astragalus membranaceus, which has antioxidant, immunomodulatory and anti-inflammatory properties. However, the therapeutic effects and biological mechanisms of APS on chemotherapeutic intestinal mucositis (CIM) have not been clarified yet. Here, the protective mechanism and functional components of APS against CIM was investigated in both Drosophila melanogaster (fruit fly) and mice models. Administration of APS could remarkably attenuate the overall physiological impairments caused by CPT-11 in flies, including increased the survival rate, improved motility, restored the size of ovary and reproduction. APS supplementation could significantly alleviate CPT-11-induced intestinal damage, which involved in restoration of intestinal length, reduction of crop size and excretion, improvement of intestinal homeostatic imbalance, and restoration of intestinal shortened villi. Furthermore, the integration of transcriptomics and microbiomics demonstrated that APS exerted its protective effect mainly by mitigating oxidative stress associated with FoxO signaling, over-activated innate immunity and dysbiosis of intestinal flora. Subsequently, three molecular weight components (APS-I, APS-II and APS-III) were extracted from APS. Among the studied substances, APS-III as the lowest molecular weight demonstrated the highest efficacy in reducing intestinal mucositis compared to both APS-I and APS-II. Collectively, these results support that APS is intended to be constructed as an effective medication for addressing intestinal diseases.