文献类型：期刊文献

中文题名：淫羊藿-黄芪调控SCF/c-kit和PI3K/Akt信号通路防治少弱精症的作用机制

英文题名：Action mechanism of Epimedii folium-Astmgali radix membranaceus regulates SCF/c-kit and PI3K/Akt signaling pathways to control oligoasthenospermia

作者：李彦荣[1];陈振东[2];张秋菊[1];蒋宜伟[3];刘光炜[3];杨扶德[4]

第一作者：李彦荣

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃中医药大学中西医结合学院,甘肃兰州730000;[3]甘肃中医药大学中医临床学院,甘肃兰州730000;[4]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2025

卷号：41

期号：9

起止页码：1693

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金资助项目(No 82360905);定西市科技计划资助项目(No DX2021AZ04)。

语种：中文

中文关键词：淫羊藿-黄芪;少弱精症;SCF/c-kit信号通路;PI3K/Akt信号通路;生精细胞;睾丸;不育症

外文关键词：Epimedii folium-Astmgali radix;oligoasthenospermia;SCF/c-kit signaling pathway;PI3K/Akt signaling pathway;spermatogenic cells;testicle;infertility

摘要：目的探讨淫羊藿-黄芪通过激活SCF/c-kit信号通路活化PI3K/Akt信号通路对少弱精症精子生成和活力的作用机制。方法SD雄性大鼠60只,随机分为实验组(中药高剂量组、中剂量组、低剂量组)、模型组、阳性对照组和空白对照组。实验组、阳性对照组和模型组用环磷酰胺建立大鼠少弱精症模型。高、中、低剂量组大鼠分别按生药淫羊藿-黄芪提取物计算量800、400、200 mg·kg^(-1)灌胃,每日1次,给药35 d。阳性对照组予以左卡尼汀250 mg·kg^(-1)·d^(-1)灌胃,每日1次,给药35 d。ELISA检测血清T、E_(2)、FSH、LH,分别用Western blot法和IHC染色检测大鼠睾丸组织中SCF、c-kit、Bcl-2、Bax蛋白表达和PI3K、Akt蛋白表达,显微镜检测精子活性、睾丸组织结构和细胞形态特点。结果与模型组相比较,淫羊藿-黄芪可使大鼠模型精子密度、总活率和活力增加(P<0.05,P<0.01),精子凋亡率和LH、T、E_(2)水平降低(P<0.05,P<0.01),睾丸组织中Bax蛋白表达降低(P<0.01),Bcl-2、SCF、c-Kit、PI3K和Akt蛋白表达升高(P<0.05,P<0.01);生精细胞数量增多,生精小管形态改善明显,界膜增厚,管中有不同发育阶段的生精细胞及精子。结论淫羊藿-黄芪对少弱精症大鼠有明显的治疗作用,其机制可能是淫羊藿-黄芪通过激活SCF/c-kit信号通路活化PI3K/Akt信号通路促使精原细胞增殖、分化使精子发生、成熟且获能。
Aim To explore the mechanism of Epimedii folium-Astmgali radix activating the SCF/c-kit signaling pathway to activate the PI3K/Akt signaling pathway and its effect on sperm production and vitality in oligoasthenospermia.Methods Sixty male SD rats were used to establish a model of oligoasthenospermia with cyclophosphamide.They were randomly divided into six groups:experimental group(further divided into high,medium,and low dose group),model group,control group and blank group.The oligoasthenospermia model was established by using cyclophosphamide in experimental group,levocarnitine group and model group.The rats in the high,medium,and low dose group of the experimental group were orally administered Epimedii folium-Astmgali radix extract at doses of 800,400,and 200 mg·kg^(-1),respectively,Once daily for 35 days.Rats of the control group were orally administered 250 mg·kg^(-1)·d^(-1) of levocarnitine,Once daily for 35 days.ELISA was used to detect serum of T,E_(2),FSH,and LH.Western blot and IHC staining were used to detect the expression of SCF,c-kit,Bcl-2,Bax,PI3K,and Akt proteins in rat testicular tissues.Sperm activity is examined by microscopy.The testicular tissue structure and cell morphology of rats in each group were observed.Results Compared with the model group,Epimedii folium-Astmgali radix increased the sperm density,total viability rate,and vitality(P<0.05,P<0.01),decreased sperm apoptosis rate and LH,T,and E_(2) levels(P<0.05,P<0.01),decreased Bax protein expression in testicular tissue(P<0.01),and increased Bcl-2,SCF,c-Kit,PI3K,and Akt protein expression(P<0.05,P<0.01);it increased the number of germ cells,thickened basement membrane,and significantly improved seminiferous tubule morphology,even showing germ cells at different developmental stages and mature sperm.Conclusions Epimedii folium-Astmgali radix has a significant therapeutic effect on oligoasthenospermia in rats.Its mechanism may be related to the activation of the SCF/c-kit signaling pathway to activate the PI3K/Akt signaling pathway promoting the proliferation and differentiation of germ cells,and promoting sperm production,maturation and motility.