文献类型：期刊文献

中文题名：红芪有效部位对肺间质纤维化模型大鼠肺组织胶原面积、透明质酸及层黏连蛋白的影响

英文题名：Effects of Active Ingredients in Radix Hedysari on Collagen Area, Hyaluronic Acid and Laminin of Lung Tissues in Rats with Pulmonary Interstitial Fibrosis

作者：苏韫[1];张毅[1];李娟[1];李雪燕[1];刘永琦[1];蔺兴遥[1];李金田[1]

第一作者：苏韫

机构：[1]甘肃中医药大学、敦煌医学与转化教育部重点实验室、中药药理与毒理学省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2016

卷号：23

期号：4

起止页码：72

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金地区科学基金(30960037/C020409);教育部春晖计划项目(Z2004-1-620039);甘肃中医学院中青年基金(2010-2)

语种：中文

中文关键词：肺纤维化;红芪有效部位;胶原面积;透明质酸;层黏连蛋白;大鼠

外文关键词：pulmonary fibrosis;;active ingredients in Radix Hedysari;;collagen area;;HA;;LN;;rats

摘要：目的观察红芪对肺间质纤维化模型大鼠肺组织胶原面积、透明质酸(HA)及层黏连蛋白(LN)表达的影响,探讨其作用机制,并筛选红芪最佳有效部位。方法将144只SPF级Wistar大鼠随机分为空白组、模型组、强的松组,以及红芪多糖、黄酮、皂苷的高、中、低剂量组。采用气管滴注博莱霉素法建立肺纤维化模型,从造模后第2日起,各药物组分别给予相应剂量药物,每日1次,共28 d。Masson染色观察肺组织胶原纤维的变化,放射免疫法检测各组大鼠肺组织匀浆中HA、LN含量。结果与模型组比较,红芪黄酮各剂量组、红芪多糖低剂量组肺泡炎明显减轻。红芪黄酮低剂量组、红芪多糖中剂量组、红芪皂苷低剂量组肺组织胶原面积明显减小。与模型组比较,红芪多糖高剂量组、红芪黄酮各剂量组、红芪皂苷高剂量组HA含量明显下降。红芪多糖高剂量组,红芪黄酮中、低剂量组,红芪皂苷中、低剂量组LN含量明显下降。结论红芪多糖、黄酮、皂苷在适宜剂量下均能减轻肺泡炎症,抑制胶原纤维增生、沉积,降低肺组织中HA、LN含量,其中红芪黄酮效果较佳。
Objective To observe the effects of Radix Hedysari on collagen area, hyaluronic acid (HA) and laminin (LN) of lung tissues in rat with pulmonary interstitial fibrosis; To investigate the mechanism and screen the best active ingredients in Radix Hedysari.Methods Totally 144 SPF Wistar rats were randomly divided into control group, model group, metacortandracin group, and Radix Hedysari polysaccharide, flavone and saponin groups were set as high-, medium-, and low-dose groups. Pulmonary interstitial fibrosis models were set up by dropping bleomycin into air tube of rats. All groups were taken corresponding medicine with appropriate dose daily on day 2 after models were established for 28 days. Collagen fibrils in lung tissue were observed by Masson dying and contents of HA and LN in lung tissue of rats in each group were detected by radioimmunoassay.Results Compared with the model group, pulmonary alveoli in Radix Hedysari flavone high-, medium-, and low-dose groups and Radix Hedysari polysaccharide low-dose group were relieved obviously; collagenous fiber area in Radix Hedysari flavone low-dose group, Radix Hedysari polysaccharide medium-dose group, and Radix Hedysari saponin low-dose group decreased obviously. Compared with the model group, the content of HA in Radix Hedysari polysaccharide high-dose group, Radix Hedysari flavone high-, medium-, and low-dose groups, and Radix Hedysari saponin high-dose group decreased obviously; the content of LN in Radix Hedysari polysaccharide high-dose group, Radix Hedysari flavone medium- and low-dose groups, and Radix Hedysari saponin medium- and low-dose groups decreased obviously. Conclusion Polysaccharide, flavone and saponin of Radix Hedysari have the abilities to alleviate inflammation of pulmonary alveoli, inhibit proliferation and deposition of collagen fibrils, and reduce the contents of HA and LA in lung tissue. Among these active ingredients, flavone has the best effect.