文献类型：期刊文献

中文题名：苓甘五味姜辛汤对哮喘大鼠环腺苷酸、环腺苷酸依赖性蛋白激酶A及水通道蛋白5的影响

英文题名：Effects of Linggan Wuwei Jiangxin Decoction on c AMP, c AMP Dependent PKA, and AQP5 of Asthma Rats

作者：李岩[1];李荣科[1];王燕[1];段云燕[1];朱向东[1]

第一作者：李岩

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2015

卷号：22

期号：10

起止页码：67

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省高校基本科研业务费项目(BH-2013-26)

语种：中文

中文关键词：支气管哮喘;苓甘五味姜辛汤;环腺苷酸;环腺苷酸依赖性蛋白激酶A;水通道蛋白5;大鼠

外文关键词：bronchial asthma;;Linggan Wuwei Jiangxin Decoction;;c AMP;;PKA;;AQP5;;rats

摘要：目的观察苓甘五味姜辛汤对寒饮伏肺型哮喘大鼠环腺苷酸(c AMP)、环腺苷酸依赖性蛋白激酶A(PKA)及水通道蛋白5(AQP5)含量的影响,探讨其对寒饮伏肺型哮喘的作用机制。方法采用卵清蛋白腹腔注射和雾化激发+寒冷刺激建立哮喘模型。将90只大鼠随机分为空白组、模型组、地塞米松组及苓甘五味姜辛汤低、中、高剂量组,各治疗组给予相应药物干预,ELISA检测大鼠c AMP、PKA、AQP5含量。结果与空白组比较,模型组大鼠c AMP、PKA、AQP5含量降低(P<0.05);与模型组比较,各治疗组大鼠c AMP、PKA、AQP5含量升高(P<0.05),其中苓甘五味姜辛汤高剂量组作用显著(P<0.05)。结论苓甘五味姜辛汤可调节寒饮伏肺型哮喘大鼠c AMP、PKA、AQP5正常分泌,增加气道液体分泌,降低黏蛋白浓度,从而起到治疗哮喘的作用。
Objective To observe the effects of Linggan Wuwei Jiangxin Decoction on contents of c AMP, c AMP dependent PKA and AQP5 of model rats with clod phlegm-fluid lying latent in the lung asthma;To discuss its mechanism. Methods Ovalbumin intraperitoneal injection and atomization motivation + cold stimulation were used to establish models. Ninety rats were randomly divided into blank control group, model group, hexadecadrol group, and Linggan Wuwei Jiangxin Decoction low-, medium-, high-dose groups. All administration groups were treated with relevant medicine. The levels of c AMP, PKA, and AQP5 were detected by ELISA. Results Compared with the blank control group, the levels of c AMP, PKA, and AQP5 of rats in model group decreased obviously(P <0.05). Compared with the model group, the levels of c AMP, PKA, and AQP5 of rats in all administration groups increased(P < 0.05), which increased dramatically in Linggan Wuwei Jiangxin Decoction high-dose group. Conclusion Linggan Wuwei Jiangxin Decoction can regulate the normal secretion of c AMP, PKA, and AQP5 in serum of rats with clod phlegm-fluid lying latent in the lung asthma, increase secretion of air passage liquid and decrease mucoprotein concentration, through which plays the role of treating asthma.