文献类型：期刊文献

中文题名：载双黄连粉针剂骨水泥珠链局部治疗对慢性骨髓炎模型兔血清CRP,TNF-α,IL-6的影响

英文题名：Influence on serum CRP,TNF-α and IL-6 with local treatment of bone cement beads contained Shuanghuanglian Fenzhenji in rabbits with chronic osteomyelitis

作者：曾昭洋[1];胡文斌[2];李洪志[3];强胜林[4];田洹[5];毛艳军[5];梁恬[2]

第一作者：曾昭洋

机构：[1]甘肃中医药大学中医临床学院;[2]青海大学附属医院康复医学科;[3]金昌市中西医结合医院骨科;[4]甘肃中医药大学附属医院创伤骨科;[5]甘肃中医药大学附属医院检验科

第一机构：甘肃中医药大学中医临床学院

年份：2017

卷号：34

期号：2

起止页码：1

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：甘肃省自然科学基金项目(1107RJ2A134)

语种：中文

中文关键词：慢性骨髓炎;双黄连粉针剂;骨水泥珠链;C-反应蛋白;肿瘤坏死因子-α;白细胞介素-6;实验研究

外文关键词：chronic osteomyelitis ; Shuanghuanglian Fenzhenji ; bone cement beads ; CRP ; TNF-α ; IL-6 ; experi- mental study

摘要：目的初步评价载双黄连粉针剂骨水泥珠链局部治疗慢性骨髓炎兔模型的有效性。方法将58只健康纯种新西兰大白兔根据骨水泥缓释系统载药不同随机分为正常组、模型组、万古霉素组、低浓度双黄连粉针剂组、高浓度双黄连粉针剂组、低浓度双黄连粉针剂与万古霉素联合组、高浓度双黄连粉针剂与万古霉素联合组,先造模再分组治疗。分别在治疗前,治疗后第2周、第4周、第8周心脏采血,观测兔血清C-反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)与白细胞介素-6(IL-6)的含量变化。结果在治疗全程,与正常组比较,模型组兔血清CRP,TNF-α,IL-6含量显著升高,差异均有高度统计意义(P<0.01);与模型组比较,各治疗组兔血清CRP,TNF-α含量显著降低,差异均有统计意义(P<0.05),治疗后第8周时,各治疗组兔血清CRP,TNF-α含量均明显降低,其中以低浓度双黄连粉针剂与万古霉素联合组、高浓度双黄连粉针剂与万古霉素联合组降低更为明显(P<0.05)。结论载高浓度双黄连与万古霉素制作的缓释系统局部治疗慢性骨髓炎兔模型效果优于载万古霉素缓释系统。
Objective To evaluate the effectiveness of bone cement beads contained Shuanghuanglian Fenzhenji in treating rabbit models with chronic osteomyelitis locally. Methods Based on drug delivery system ,58 healthy purebred New Zealand rabbit models were randomly divided into 6 groups： control group, model group, van- comycin group, low concentration of Shuanghuanglian Fenzhenji group, high concentration of Shuanghuanglian Fenzhenji group,low concentration of Shuanghuanglian Fenzhenji combining with vancomycin group, high concentra- tion of Shuanghuanglian Fenzhenji combining with vancomycin group. Before treatment, on 2 ,4th and 8th week after treatment, blood was collected from heart and changes of serum CRP, TNF-α and IL-6 were observed. Results In the course of treatment,compared with control group, contents of CRP,TNF-α and IL-6 in model group increased with significant statistical difference （P〈0.01） ;compared with model group ,contents of CRP ,TNF-α decreased in all treatment groups,the difference was significant （P〈0.05）. On 8th week after treatment,contents of CRP,TNF- α decreased in all treatment groups, especially in the two combined groups （ P〈0.05 ）. Conclusion High concen- tration of Shuanghuanglian Fenzhenji combining with vancomycin is superior to single vancomycin when they are used as drug delivery system to treat chronic osteomyelitis.