文献类型：期刊文献

中文题名：当归干、鲜品中游离阿魏酸和总阿魏酸含量

英文题名：Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh Angelica sinensis

作者：林丽[1];晋玲[1];李应东[1];侯嘉[1];高素芳[1];杨建成[1]

第一作者：林丽

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2012

卷号：18

期号：22

起止页码：94

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：国家自然基金面上项目(31070352)

语种：中文

中文关键词：HPLC法;当归;鲜品;干品;游离阿魏酸;总阿魏酸

外文关键词：HPLC; fresh Angelica sinensis Radix; dried Angelica sinensis Radix; free ferulic acid; total ferulic acid

摘要：目的:采用HPLC测定当归鲜品与干品中游离阿魏酸和总阿魏酸的含量,并对其进行比较。方法:ZORBAX EclipseXDB-C18(4.6 mm×250 mm,5μm)分析柱,流动相乙腈-0.085%磷酸(17∶83),检测波长316 nm,柱温35℃,流速1.0 mL·min-1,进样量为10μL。测定样品中游离阿魏酸和总阿魏酸的含量。结果:游离阿魏酸和总阿魏酸分别在0.024 2～0.121 0μg和0.0242～0.192 36μg呈良好的线性关系。回收率分别为96.34%,97.35%;09年当归干品中游离阿魏酸含量最高,鲜品中总阿魏酸含量最高。结论:该方法准确简便,具有相对良好的重复性和稳定性,有利于提高当归药材的质量控制。
Objective： The free and total ferulic acid content in dried and fresh Angelica sinensis Radix was measured by HPLC. Method： Free and total ferulic acid content was determined by HPLC. The separation was performed on a ZORBAX Eclipse XDB-C18 column （4.6mm×250mm,5μm） with acetonitrile-0. 085% phosphoric acid （17：83 ） as mobile phase at temperature of 35℃. The flow rate was 1.0mL·min^-1 and the detected wavelength was set at 316 nm; the injection volume was 10 μL. Result： The linear relationship of free and total ferulic acid was exceilent within the range of 0. 024 2-0. 121 0 p^g and 0. 024 2-0. 192 36 μg, and the recovery rates were 96. 34% and 97.35% , respectively. The free ferulic acid in dried A. sinensis Radix of 2009 was the highest and the total ferulic acid in fresh A. sinensis Radix was the highest. Conclusion： This method was simple and accurate with good repeatability and stability. It Will help to improve the quality control of A. sinensis Radix.