文献类型：期刊文献

中文题名：基于体素的磁共振形态学腰椎间盘突出症慢性下肢痛模型大鼠的实验研究

英文题名：Chronic lower extremity pain due to lumbar disc herniation:an experimental study in rats using voxel-based magnetic resonance morphometry

作者：赵继荣[1];薛旭[1];袁毓喆[2];陈祁青[1];陈文[1];张海清[1];赵宁[1];马同[1];张天龙[3];杨涛[3]

第一作者：赵继荣

机构：[1]甘肃省中医院脊柱骨科,甘肃兰州730050;[2]中国药科大学药学院,江苏南京210000;[3]甘肃中医药大学研究生院,甘肃兰州730030

第一机构：甘肃省中医院脊柱骨科,甘肃兰州730050

年份：2021

卷号：38

期号：7

起止页码：820

中文期刊名：中国医学物理学杂志

外文期刊名：Chinese Journal of Medical Physics

收录：CSTPCD;;CSCD:【CSCD_E2021_2022】；

基金：国家自然科学基金(81760877);兰州市人才创新创业专项(2018-RC-99)。

语种：中文

中文关键词：腰椎间盘突出症;磁共振形态学;机械缩足反射阈值;热缩足反射潜伏期;疼痛

外文关键词：lumbar disc herniation;magnetic resonance morphometry;mechanical withdrawal threshold;thermal withdrawal latency;pain

摘要：目的:利用基于体素的磁共振形态学(VBM)和疼痛行为学观察腰椎间盘突出症(LDH)慢性下肢痛大鼠模型脑结构及形态改变的相关性及特点,揭示LDH慢性下肢痛的脑机制。方法:选用SD雄性大鼠24只,6~8周龄,体质量(250±20)g,随机分为3组,即正常组(Normal组)、假手术组(Sham-LDH组)和模型组(LDH组),采用自体髓核移植法建立LDH慢性下肢痛模型大鼠。各组分别于建模前和建模后第2、7、14、21、28天行疼痛行为学测定,主要包括机械缩足反射阈值(MWT)及热缩足反射潜伏期(TWL)的测定。每组随机选取4只大鼠分别于建模前和建模后第14、28天行T2加权结构图像和VBM图像采集,采用重复测量方差分析对灰质体积进行统计学分析。结果:3组大鼠建模前MWT比较,差异无统计学意义(P>0.05);Sham-LDH组大鼠MWT建模后第2、7、14、21、28天分别与Normal组大鼠比较,差异无统计学意义(P>0.05);LDH组大鼠MWT从建模后第2天至第14天显著降低,建模后第14天至28天,MWT稳定;LDH组大鼠MWT建模后第2、7、14、21、28天分别与Normal组大鼠和Sham-LDH组大鼠比较,差异有统计学意义(P<0.05)。TWL分析结果与MWT分析结果相似。3组大鼠不同时间点脑结构区域比较存在交互作用,差异具有统计学意义(P<0.05),具体表现为左下丘脑、左海马下托、右次级运动皮层、右纹状体、右隔区、右海马、胼胝体及双侧皮质下灰质、双侧小脑分子层这些脑区的灰质体积减少。结论:成功建立自体髓核移植型LDH慢性下肢痛大鼠模型,造模后产生一个长时程的MWT和TWL的降低,出现痛觉过敏,造模成功。利用VBM能够确切地发现LDH慢性下肢痛模型大鼠脑结构发生了形态学改变。
Objective To study the correlation and characteristics of brain structural and morphological changes in rat models of chronic lower limb pain due to lumbar disc herniation(LDH)using voxel-based magnetic resonance morphometry(VBM)and pain behavioral observation,thereby revealing the brain mechanism of LDH chronic lower limb pain.Methods Twenty-four SD male rats aging 6-8 weeks old,with a body weight of(250±20)g,were selected as the research objects and randomly divided into 3 groups,namely Normal group,Sham-LDH group and LDH group;and the rat model of LDH chronic lower limb pain was established by the transplantation of autologous nucleus pulposus.The pain behavioral observation was performed in each group before modeling and on the 2nd,7th,14th,21st and 28th days after modeling,mainly including the measurements of mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL).Four rats in each group were randomly selected to collect T2-weighted structural images and VBMimages before modeling and on the 14th and 28th days after modeling.Repeated measures analysis of variance was used for the statistical analysis of gray matter volume.Results There was no significant difference in MWT among 3 groups before modeling,without statistical differences(P>0.05).The MWT in Sham-LDH group and Normal group was similar on the 2nd,7th,14th,21st and 28th days after modeling(P>0.05).In LDH group,MWT decreased significantly from the 2nd day to the 14th day after modeling,and then remained stable from the 14th day to the 28th day after modeling.On the 2nd,7th,14th,21st and 28th days after modeling,the MWT in LDH group was statistically different from that in Normal group and Sham-LDH group(P<0.05).The comparison results of TWL were similar to those of MWT.There were interactions between the rat brain structures of 3 groups at different time points,and the differences were statistically significant(P<0.05).Gray matter volume was reduced in some brain regions,specifically,left hypothalamic region,left hippocampus subiculum,right secondary motor cortex,right striatum,right septal region,right cornu ammonis,corpus callosum and associated subcortical gray matter,molecular layer of the lateral cerebellum.Conclusion Arat model of LDH chronic lower limb pain is successfully established after the transplantation of autologous nucleus pulposus.Long-term reductions of MWT and TWL emerge and the hyperalgesia occurs after the modeling.The morphological changes in the brain structures of rat models of LDH chronic lower limb pain can be found using VBM.