文献类型：期刊文献

英文题名：Material Basis of the Difference between Hedysari Radix and Honey-Processed Hedysari Radix in Buzhong Yiqi

作者：Niu, Jiang-tao[1,2];Cao, Rui[1,2];Si, Xin-lei[1,2];Bian, Tian-tian[1,2];Xin, Er-dan[1,2];Li, Yue-feng[1,2];Yan, Xing-ke[3]

第一作者：Niu, Jiang-tao

通信作者：Li, YF[1];Li, YF[2];Yan, XK[3]

机构：[1]Gansu Univ Chinese Med, Pharm Coll, Lanzhou 730000, Peoples R China;[2]Key Lab Qual & Stand TCM Gansu Prov, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Acupuncture Coll, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Pharm Coll, Lanzhou 730000, Peoples R China;[2]corresponding author), Key Lab Qual & Stand TCM Gansu Prov, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Acupuncture Coll, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2020

卷号：2020

外文期刊名：EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE

收录：;Scopus(收录号：2-s2.0-85097738884);WOS:【SCI-EXPANDED(收录号:WOS:000599830700004)】；

基金：This work was supported by the National Natural Science Foundation of China (no. 81960713), the Gansu Natural Science Foundation Innovation Base and Talent Program (18JR3RA197), and the Gansu Provincial Key Laboratory of Quality and Standards for Traditional Chinese Medicine (ZYZL18-008).

语种：英文

摘要：Objective. To compare the changes of chemical components of Hedysari Radix (HR) before and after honey-processing, and to explore the material basis of the difference between HR and honey-processed Hedysari Radix (HPHR) in Buzhong Yiqi. Methods. Different compounds in aqueous extracts of HR and HPHR were analysed by UPLC-MS. A rat model of spleen qi deficiency was established. The rats were treated with different doses of water extracts of HR or HPHR, and pathological differences in spleen tissue, serum levels of D-xylose, gastrin (GAS) and amylase (AMS) interleukin-2 (IL)-2 and tumour necrosis factor-alpha (TNF-alpha), as well as spleen and thymus indices, were used as indicators. Differences in the efficacy of HR and HPHR in Buzhong Yiqi were studied. Results. The research showed that compared with the blank group, the spleen tissue of rats in the model group showed spleen tissue damage, which mainly manifested as unclear boundaries between red pulp and white pulp, irregular spleen morphology and irregular arrangement, and the structure of white pulp destruction, less lymphocytes, the number of germinal centers decreased or atrophied. Compared with the model group, the middle and high dose groups of HR and HPHR had protective effects on spleen tissue of spleen-qi deficiency rats, and HPHR had a stronger effect; compared with those in the model group, rats in each treatment group showed remarkably higher serum D-xylose, GAS and AMS levels and thymus and spleen indices, and remarkably lower serum IL-2 and TNF-alpha levels, among which HPHR group showed better regulation effect than HR group. A total of 16 differential compounds were found in the aqueous extracts of HR and HPHR, of which 10 compounds in HPHR were up regulated, while 6 compounds were down regulated compare to HR. Conclusion. The results indicated that both HR and HPHR can improve spleen qi deficiency syndrome of rats, the pharmacodynamic effect of the latter was better than the former. Differences in components of HR and HPHR potentially leading to variations in efficacy.