文献类型：期刊文献

中文题名：雌激素对大鼠内脏痛觉感受及结肠5-羟色胺合成的影响

英文题名：EFFECTS OF ESTROGEN ON THE VISCERAL PAIN SENSITIVITY AND COLONIC 5-HT SYNTHESIS IN RATS

作者：臧凯宏[1,2];秦红岩[3];马骏[1,2];任远[1,2];朱丽娟[1,2]

第一作者：臧凯宏

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省中药药理与毒理学重点实验室,兰州730000;[3]兰州大学第一医院药剂科,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：23

期号：9

起止页码：651

中文期刊名：中国疼痛医学杂志

外文期刊名：Chinese Journal of Pain Medicine

收录：CSTPCD;;CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81341145;81400596)

语种：中文

中文关键词：雌激素;内脏痛;5-羟色胺;卵巢切除;嗜铬细胞

外文关键词：Estrogen;Visceral pain;5-Hydroxytryptamine;Ovariectomy;Enterochromaffin cells

摘要：目的:研究雌激素对大鼠内脏痛觉感受及结肠5-羟色胺(5-hydroxytryptamine,5-HT)合成的影响。方法:Wistar雌性大鼠采用随机数字表分组,分为假手术组,卵巢切除组及卵巢切除+雌激素组。卵巢切除+雌激素组动物皮下注射苯甲酸雌二醇(30μg·kg^(-1)),连续给药2周。给药结束后次日测定大鼠内脏痛阈值、血清雌激素和孕激素水平;收集结肠组织测定5-HT含量、嗜铬细胞数量及色氨酸羟化酶的表达。结果:雌激素替代给药后显著提高卵巢切除大鼠血清雌激素水平、降低内脏痛阈值、明显提高结肠嗜铬细胞数量、5-HT含量及色氨酸羟化酶的表达。结论:雌激素对结肠5-HT合成具有正性调控作用,该机制可能参与雌激素介导的内脏痛觉感受增强。
Objective：To investigate the effects of estrogen on visceral pain sensitivity and colonic 5-hydroxytryptamine （5-HT） synthesis in rats. Methods： Female Wistar rats were randomly divided and received ovariectomy （OVX） or sham operation. Three groups were set for this study, they are sham group, OVX group and OVX plus estrogen supplementation group. Rats in OVX group and sham group were treated with pure sesame oil subcutaneously as controls, and rats in OVX plus estrogen group were subcutaneously treated with estradiol benzoate （30 ug？kg-1）, drugs were given to rats for consecutive 2 weeks. After drugs treatment, pain threshold pressure was measured by abdominal withdrawal reflex test; blood was collected for determination of the serum level of estrogen and progesterone by ELISA; and proximal colon tissue was also collected for 5-HT content assay, enterochromaffin cells counting, and tryptophan hydroxylase （TPH） expression detection by ELISA, immunohistochemistry and Western-blot, respectively. Results： Compared with the rats in OVX group, the pain threshold in estrogen-treated rats was marked decreased, while colonic enterochromaffin cells density, 5-HT content as well as TPH expression were all significantly increased. Conclusion： Estrogen can positively regulate colonic 5-HT synthesis, and this effect may have correlation with estrogen-mediated increase of visceral sensitivity.