文献类型：期刊文献

英文题名：Baicalin extracted from Huangqin (Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma (Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9

作者：Wang Hongwei[1,2];Li Hailong[1,2,3,4];Chen Fengqin[2];Luo Jun[2];Gu Jing[2];Wang Huping[5];Wu Hongyan[5];Xu Yan[2]

第一作者：Wang Hongwei;王宏伟

通信作者：Xu, Y[1]

机构：[1]Gansu Univ Chinese Med, Hosp, Clin Lab, Lanzhou 730000, Peoples R China;[2]Key Lab Transfer Dunhuang Med Prov & Minist Level, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Sch Clin Med, Dept Teaching, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Sch Clin Med, Res Clin Lab, Lanzhou 730000, Peoples R China;[5]Gansu Univ Chinese Med, Gansu Prov Key Lab Chinese Med Herbs Prescript In, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2017

卷号：37

期号：2

起止页码：229

外文期刊名：JOURNAL OF TRADITIONAL CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000401206500012)】；

基金：Supported by the Natural Science Foundation of Gansu Province (No. 148RJZA073); the Health Industry Scientific Research Project of Gansu Province (No. GWGL 2013-40); and the Openning Plan Project of Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and Ministerial Level (No. DHYX1213-008)

语种：英文

外文关键词：Baicalin; Stomach neoplasms; Apoptosis-Lymphoma, B-Cell; Bcl-2-associated X protein; Caspases, effector

摘要：OBJECTIVE: To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action. METHODS: Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells. Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin. RESULTS: In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 mu mol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner. Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and.B cell lymphoma (Bcl-2)-associated X protein and down-regulated the expression of BcI-2 at both the mRNA and protein level. CONCLUSION: Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells. (C) 2017 JTCM. All rights reserved.