文献类型：期刊文献

英文题名：The Influence of the Total Flavonoids of Hedysarum Polybotry on the Proliferation, Cell Cycle, and Expressions of p21^Ras and Proliferating Cell Nuclear Antigen Gene in Erythroleukemia Cell Line K562

作者：Wang Ya-li[1];Luo Ya-li[1];Chen Che[1];Li Neng-lian[1];She Ya-li[1];Zhang Li[1]

第一作者：王亚利;王亚莉;汪亚莉;王亚丽

通信作者：Wang, YL[1]

机构：[1]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2012

卷号：18

期号：5

起止页码：385

外文期刊名：CHINESE JOURNAL OF INTEGRATIVE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000303362100011)】；

基金：Supported by the Natural Science Foundation for Middle-Aged and Young Scientist of Gansu Province (No. Y5031-A21-015)

语种：英文

外文关键词：total flavonoids of Hedysarum polybotry; K562 cells; cell cycle; p21(Ras) and proliferating cell nuclear antigen

摘要：Objective: To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21(Ras) and proliferating cell nuclear antigen (PCNA) gene in erythroleukemia cell line K562. Methods: The effect of total flavonoids of Hedysarum polybotry on K562 cell line survival was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay. The time- and dose-dependent manner was also observed. The cell cycle and apoptosis were analyzed with flow cytometry (FCM). The immunocytochemistry method was applied to quantitatively analyze the effects of flavonoids of Hedysarum polybotry on changes p21(Ras) and PCNA gene expressions. Results: Flavonoids of Hedysarum polybotry (20-100 mu g/mL) significantly inhibited the proliferation of K562 cells in a time- and dose-dependent manner. After K562 cells were cultured for 48 h, total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition (P<0.01), indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at G(0)/G(1) and G(2)/M phases. Compared with the control group, p21(Ras) and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry (40 and 80 mu g/mL, respectively) for 48 h. Conclusion: The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry, which might be related to cells arresting.