文献类型：期刊文献

英文题名：Protective effect of San Huang Pill and its bioactive compounds against ulcerative colitis in Drosophila via modulation of JAK/STAT, apoptosis, Toll, and Nrf2/Keap1 pathways

作者：Li, Botong[1,2];Xiu, Minghui[3,4];He, Li[3];Zhou, Shihong[3];Yi, Simeng[5];Wang, Xiaoqian[1,2];Cao, Wangjie[1,2];Liu, Yongqi[1,4];He, Jianzheng[1,2,4,6]

第一作者：Li, Botong

通信作者：Liu, YQ[1];He, JZ[1]

机构：[1]Gansu Univ Chinese Med, Prov level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Coll Basic Med, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial L, Lanzhou 730000, Peoples R China;[5]China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr & Feeding, Beijing 100193, Peoples R China;[6]Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumor, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Prov level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：322

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85181022896);WOS:【SCI-EXPANDED(收录号:WOS:001146671300001)】；

基金：This work was supported by the Provincial University industry support project in Gansu (No. 2020C-15) , National Natural Science Foundation of China (No. 81960793) , Gansu Provincial Natural Science Foundation (No. 23JRRA1202) , Gansu Longyuan Youth Talent Innovaion and Entrepreneurship Project, Lanzhou Science and Technology Plan Project (No. 2023-4-53) .

语种：英文

外文关键词：San huang pill; Dunhuang ancient medical prescription; Ulcerative colitis; Drosophila melanogaster; Signaling pathway; Bioactive compounds

摘要：Ethnopharmacological relevance: San Huang Pill (SHP) is a prescription in Dunhuang Ancient Medical Prescription, which has the efficacy of heat-clearing and dampness-drying, and is a traditional formula for the treatment of gastrointestinal diseases. However, its efficacy and mechanism in treating ulcerative colitis (UC) are still unclear.Aim of the study: To investigate the protective effects of SHP and its bioactive compounds against Dextran Sulfate Sodium (DSS)-induced intestinal damage using the Drosophila melanogaster model, and to detect the molecular mechanism of SHP in the treatment of UC. Methods: Survival rate, locomotion, feeding, and excretion were used to explore the anti-inflammatory effects of SHP. The pharmacotoxicity of SHP was measured using developmental analysis. Intestinal integrity, intestinal length, intestinal acid-base homeostasis, and Tepan blue assay were used to analyze the protective effect of SHP against DSS-induced intestinal damage. The molecular mechanism of SHP was detected using DHE staining, immunofluorescence, real-time PCR, 16 S rRNA gene sequencing, and network pharmacology analysis. Survival rate, intestinal length, and integrity analysis were used to detect the protective effect of bioactive compounds of SHP against intestinal damage.Results: SHP supplementation significantly increased the survival rate, restored locomotion, increased metabolic rate, maintained intestinal morphological integrity and intestinal homeostasis, protected intestinal epithelial cells, and alleviated intestinal oxidative damage in adult flies under DSS stimulation. Besides, administration of SHP had no toxic effect on flies. Moreover, SHP supplementation remarkably decreased the expression levels of genes related to JAK/STAT, apoptosis, and Toll signaling pathways, increased the gene expressions of the Nrf2/ Keap1 pathway, and also reduced the relative abundance of harmful bacteria in DSS-treated flies. Additionally, the ingredients in SHP (palmatine, berberine, baicalein, wogonin, rhein, and aloeemodin) had protection against DSS-induced intestinal injury, such as prolonging survival rate, increasing intestinal length, and maintaining intestinal barrier integrity.Conclusion: SHP had a strong anti-inflammatory function, and remarkably alleviated DSS-induced intestinal morphological damage and intestinal homeostatic imbalance in adult flies by regulating JAK/STAT, apoptosis, Toll and Nrf2/Keap1 signaling pathways, and also gut microbial homeostasis. This suggests that SHP may be a potential complementary , alternative medicine herb therapy for UC, which provides a basis for modern pharmacodynamic evaluation of other prescriptions in Dunhuang ancient medical prescription.