文献类型：期刊文献

中文题名：18β-甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞紧密连接的影响

英文题名：The effect of 18β-glycyrrhetinic acid on tight junctions of the nasal mucosa epithelial cells in rat models with allergic rhinitis

作者：马毅[1];桂岩[1,2];王有虎[1,2];席克虎[1,2];陈小婉[1,2];张福宏[1,2];马春霞[1];洪浩[1];刘翔毅[2];江英[1];董明[1];杨贵军[1];张小兵[1,2]

第一作者：马毅

机构：[1]兰州大学第一医院耳鼻咽喉头颈外科,兰州730000;[2]甘肃中医学院附属医院耳鼻咽喉头颈外科

第一机构：兰州大学第一医院耳鼻咽喉头颈外科,兰州730000

年份：2014

卷号：28

期号：20

起止页码：1590

中文期刊名：临床耳鼻咽喉头颈外科杂志

外文期刊名：Journal of Clinical Otorhinolaryngology Head And Neck Surgery

收录：MEDLINE(收录号：25764760);CSTPCD;;Scopus;北大核心:【北大核心2011】；PubMed;

基金：国家自然科学基金项目(No:81160449);甘肃省卫生厅行业科技计划项目(No:GSWST2011-06);甘肃省中医药科学技术研究课题(No:GZK-2011-22)

语种：中文

中文关键词：18β-甘草次酸;鼻炎,变应性;紧密连接

外文关键词：18β-glycyrrhetinic acid; rhinitis; allergic; tight junctions

摘要：目的:观察18β-甘草次酸(GA)对变应性鼻炎(AR)大鼠鼻黏膜上皮细胞紧密连接(TJs)超微结构的影响。方法:96只Wistar大鼠随机分为对照组、模型组、氯雷他定组、GA组,每组24只。卵清蛋白致敏建立大鼠AR模型,观察和比较给药干预后第2、4、6、10周,各组行为改变及透射电镜下鼻黏膜上皮细胞TJs形态。结果:AR模型TJs长度变短,质膜电子高密度影变厚、融合点数量减少,间隙增宽甚至断裂。在变应原持续作用下,模型组鼻黏膜上皮细胞TJs的上述改变呈进行性加重,而GA组TJs变短、质膜影变厚、膜融合点减少均较模型组缓解,并随观察周期延长基本接近对照组。结论:18β-GA可缓解AR大鼠鼻腔黏膜上皮细胞间TJs的损害。
Objective：To observe 18β-glycyrrhetinic acid（GA）impact on ultrastructure of tight junctions（TJs）of nasal mucosa epithelial cells in rats models of allergic rhinitis（AR）.Method：Ninety-six Wistar rats were randomly divided into control group,model group,loratadine group,and 18β-glycyrrhetinic acid group,and each group had 24 rats.Ovalbumin was used to establish a rat AR model.The behavioral changes and the tight junctions of nasal epithelial were observed and compared in different groups after 2,4,6and 10 weeks intervention.Result：The length of TJs in allergic rhinitis model became shorter,electron-high-density plasma membrane became thicker,number of the integration loci reduced and gap of TJs widened or even ruptured.With the consistent effect of allergens,the changes of TJs in the model group aggravated gradually,and the changes of ultrastructure of TJs in 18β-glycyrrhetinic acid group was relieved apparently compared to model group and even were close to the control model with time.Conclusion：18β-glycyrrhetinic acid can recover the ultrastructure of the tight junctions of AR rat nasal epithelial cells.