文献类型：期刊文献

英文题名：Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles

作者：Liu, Yong-Qi[1,4,5];Li, Yi[1,2];Qin, Jie[2,3];Wang, Qian[2];She, Ya-Li[2,4];Luo, Ya-Li[2,4];He, Jian-Xin[2];Li, Jing-Ya[2];Xie, Xiao-Dong[1]

第一作者：Liu, Yong-Qi

通信作者：Xie, XD[1]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China;[2]Gansu Tradit Chinese Med Univ, Inst Syst Biol & TCM Transformat, Lanzhou, Peoples R China;[3]Lanzhou Univ Hosp, Lanzhou, Peoples R China;[4]Minist Educ, Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou, Peoples R China;[5]Minist Educ, Key Lab Transfer Dunhuang Med Prov & Minist Level, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.

年份：2014

卷号：15

期号：5

起止页码：2169

外文期刊名：ASIAN PACIFIC JOURNAL OF CANCER PREVENTION

收录：;Scopus(收录号：2-s2.0-84899075812);WOS:【SCI-EXPANDED(收录号:WOS:000335651200046)】；

基金：We are grateful to all other members in our research group for their support and advice in regards to this study. The study was supported by The National Natural Science Foundation of China (81060351/H2810, 81272454).

语种：英文

外文关键词：Matrine; A549 lung cancer cells; miRNA profile; apoptosis; proliferation

摘要：Matrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles. In the MTT assay, matrine suppressed growth of human lung cancer cell A549 in a dose- and time-dependent manner at doses of 0.25-2.5 mg/ml for 24h, 48h or 72h. Matrine induced cell cycle arrest in G0/G1 phase and decreased the G2/M phase, while down-regulating the expression of Bcl2 protein, leading to a reduction in the Bcl-2/Bax ratio. In addition, matrine down regulated the expression level of VEGF and HDAC1 of A549 cells. Microarray analysis demonstrated that matrine altered the expression level of miRNAs compared with untreated control A549 cells. In conclusion, matrine could inhibit proliferation of A549 cells, providing useful information for understanding anticancer mechanisms.