文献类型：期刊文献

中文题名：甘肃产不同生态型板蓝根对小鼠抗炎、免疫调节作用的比较

英文题名：Comparative study on anti-inflammatory and immunomodulation of different Isatidis Radix strains growing in Gansu Province

作者：赵泽军[1];王志旺[1];郭玫[1];杜弢[1];师霞[1];邵晶[1];文喜艳[1]

第一作者：赵泽军

机构：[1]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：34

期号：1

起止页码：57

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：甘肃省中医药管理局科研基金项目(GZK-2015-5)

语种：中文

中文关键词：板蓝根;生态型;四倍体;抗炎;免疫调节作用;小鼠

外文关键词：Isatidis Radix;ecotype;tetraploid;anti-inflammatory;immunomodulation;mice

摘要：目的:研究甘肃产不同生态型板蓝根对小鼠抗炎、免疫调节的作用。方法:66只KM小鼠随机分6组(n=11),采用耳廓肿胀法、足肿胀法观察板蓝根的抗炎作用;77只KM小鼠随机分7组(n=11),通过复制气囊滑膜炎动物模型来观测板蓝根的抗炎作用;66只KM小鼠随机分6组(n=11),通过环磷酰胺复制免疫低下小鼠模型,研究板蓝根对动物胸脾指数、血常规及细胞因子的影响。结果:甘肃产不同生态型板蓝根可降低小鼠耳廓肿胀度、足肿胀度以及气囊滑膜炎总蛋白、白三烯B4(LTB4)及丙二醛(MDA)的水平,提高气囊滑膜炎动物血清中超氧化物歧化酶(SOD)水平;降低环磷酰胺模型小鼠血清肿瘤坏死因子-α(TNF-α)水平,提高胸脾指数、红细胞与白细胞计数以及γ-干扰素(IFN-γ)、白介素-4(IL-4)水平(P<0.05,P<0.01);在上述抗炎、调节免疫的研究中,甘肃产不同生态型板蓝根及四倍体板蓝根之间未出现明显的差异。结论:甘肃产不同生态型板蓝根对小鼠具有明显的抗炎、免疫调节作用,甘肃产不同生态型板蓝根及四倍体板蓝根之间上述作用无明显差异。
Objective： To investigate anti-inflammatory and immunomodulation effects of different ecotype from Isatidis Radix growing in Gansu province. Methods： Mice were randomly divided into 6 groups（ n = 11） and used the auricular swelling and paw edema to observe the anti-inflammatory effects of Isatidis Radix; Mice were randomly divided into 7 groups（ n = 11） and through the gasbag synovitis model to observe the anti-inflammatory effects of Isatidis Radix; Mice were randomly divided into 6 groups（ n = 11）,the immunosuppressed model were established by injection of cyclophosphamide（ CTX） to study the effects of Isatidis Radix on index of thymus,blood routine and cytokines. Results： Gansu different ecotype from Isatidis Radix could reduce the swelling of the mice auricle,paw edema and total protein,leukotriene B4（ LTB4） and malonaldehyde（ MDA） in airbag synovitis exudates,and upgrade serum levels of superoxide dismutase（ SOD）; Degrade the tumor necrosis factor-α（ TNF-α） and upgrade the index of thymus,the number of red and white corpuscles,the level of interferon-γ（ IFN-γ）,interleukin-4（ IL-4）（ P〈0.05,0.01） of mice immunosuppressed model;Above the research of anti-inflammatory and immunomodulation,there were no significant differences between Isatidis Radix of Gansu different ecotype and tetraploid. Conclusion： Different ecotype of Isatidis Radix has obvious functions in anti-inflammatory and immunomodulation,but there are no significant differences between Gansu different ecotype and tetraploid.