文献类型：期刊文献

中文题名：颊针疗法对类风湿性关节炎兔的镇痛时效及其作用机制

英文题名：Analgesic effect of buccal acupuncture on acute arthritis in rabbits and underlying mechanisms

作者：颉旺军[1];方晓丽[1];蒲瑞生[2];苏成红[1];张延菊[1];蒲永乐[1];杨晨光[1]

第一作者：颉旺军

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]天水市中医医院针灸科,甘肃天水741000

第一机构：甘肃中医药大学针灸推拿学院

通信机构：[1]College of Acupuncture and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou, 730000, China

年份：2017

卷号：42

期号：5

起止页码：517

中文期刊名：中南大学学报（医学版）

外文期刊名：Journal of Central South University ：Medical Science

收录：CSTPCD;;Scopus(收录号：2-s2.0-85021691402);北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；PubMed(收录号：28626096);

基金：国家自然科学基金(81260559)~~

语种：中文

中文关键词：针刺疗法;类风湿性关节炎;颊针;β-内啡肽;八肽胆囊收缩素;大耳白兔

外文关键词：acupuncture therapy; rheumatoid arthritis; buccal acupuncture; β-endorplhin; cholecystokinin-8;flap-eared white rabbits

摘要：目的:探究针刺对患有类风湿性关节炎大耳白兔的镇痛时效及其中枢作用机制。方法:将60只大耳白兔随机分为正常组(n=6)、模型组(n=6)、体针组(n=24)和颊针组(n=24),再将后两组分为针后即时(0 h)、针后0.5,1,2 h 4个亚组,每组各6只。白兔类风湿性关节炎模型采用卵蛋白诱导建立。体针组针刺双侧"膝眼"和"足三里"1次,颊针组双侧颊针针刺"膝"1次,行针15 s,留针30 min。采用PL-200热刺痛仪检测痛阈,以兔出现缩耳挣扎动作为疼痛反应指标,自照射开始至兔出现反应的潜伏期时间作为该兔的痛阈值;使用放射免疫法检测兔脑脊液中β-内啡肽(β-endorplhin,β-EP)及八肽胆囊收缩素(cholecystokinin-8,CCK-8)含量。结果:与正常组相比,模型组痛阈和CCK-8含量显著降低(P<0.01),β-EP含量显著升高(P<0.05);与模型组相比,体针组和颊针组的痛阈在0和1 h(P<0.05或P<0.01)显著升高;体针组和颊针组兔脑脊液中CCK-8和β-EP含量均显著高于模型组(P<0.01或P<0.05);颊针组的即时(0 h)CCK-8和β-EP含量显著高于体针组(P<0.05),其他时间无明显变化。结论:针刺治疗可以有效缓解类风湿性关节炎的疼痛,颊针疗法的即时镇痛效应优于体针治疗,且这种即时镇痛效应与脑脊液中β-EP和CCK-8含量变化相关。
Objective： To observe the analgesic effect of acupuncture and to explore its central analgesic mechanism in rheumatoid arthritis rabbits. Methods： A total of 60 flap-eared white rabbits were randomly assigned into a normal control group （n=6）, a model group （n=6）, a body-acupuncture group （n=24）, and a buccal acupuncture group （n=24）. The later 2 groups were further randomly assigned into 0, 0.5, 1, and 2 h subgroups, with 6 cases in each group. The rheumatoid arthritis model was established by induction of eggalbumin. In the body acupuncture group, bilateral ＂Xiyan＂ and ＂Zusanli＂ were punctured for 15 s while in the buccal acupuncture group, acupuncture was applied to ＂Xi＂ for 15 s, with the needle retaining for 30 min. The pain threshold was detected with PL-200, taking struggle movements of rabbits as a measurement index, response latency from irradiation to struggling movements as the rabbit＇s pain threshold. The contents of β-endorplhin （β-EP） and cholecystokinin-8 （CCK-8） in cerebrospinal fluid were examined by radioimmunoassay. Results： Compared with the control group, pain threshold and CCK-8 levels decreased significantly （P〈0.01） and the concentration of β-EP significantly increased （P〈0.05） in the model group. The pain threshold in the body-acupuncture group and the buccal acupuncture group at 0 and 1 h （P〈0.05 or P〈0.01） increased significantly, while the β-EP and CCK-8 contents in the bodyacupuncture group and the buccal acupuncture group were significantly higher than those in the model group （P〈0.01 or P〈0.05）. Both β-EP and CCK-8 contents in the buccal acupuncture group at 0 h were significantly higher than those in the body-acupuncture group （P〈0.05）. Conclusion： The analgesic effect of buccal acupuncture is superior to that of body-acupuncture. Both buccal acupuncture and body-acupuncture can effectively raise the pain threshold in acute arthritis rabbits, which is closely associated with their effects in the up-regulation of β-EP and CCK- 8 contents in cerebrospinal fluid.