文献类型：期刊文献

中文题名：西藏不同产区长梗秦艽中龙胆苦苷和马钱苷酸含量研究

英文题名：Study on contents of gentiopicroside and loganin acid in Gentiana waltonii from different producing areas in Tibet

作者：张好科[1];付兆媛[2]

第一作者：张好科

机构：[1]白银市景泰县人民医院药剂科,甘肃景泰730400;[2]甘肃中医药大学附属医院肿瘤科,甘肃兰州730020

第一机构：白银市景泰县人民医院药剂科,甘肃景泰730400

年份：2016

卷号：33

期号：5

起止页码：24

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

语种：中文

中文关键词：长梗秦艽;龙胆苦苷;马钱苷酸;高效液相色谱;含量测定

外文关键词：Gentiana waltonii;;gentiopicroside;;loganic acid;;HPLC;;content determination

摘要：目的比较西藏不同产区长梗秦艽中龙胆苦苷和马钱苷酸含量的差异。方法收集西藏3个地区共8份长梗秦艽样品,采用高效液相色谱(HPLC)法对其中龙胆苦苷和马钱苷酸的含量进行测定。色谱柱为Kromasil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-0.1%磷酸水溶液,梯度洗脱,流速为1m L/min,检测波长245 nm,柱温35℃,进样量10μL。结果龙胆苦苷回归方程为Y=1 050X+34.6,r=0.999 7,结果表明龙胆苦苷在61.063~977.000μg/m L范围内线性关系良好;马钱苷酸回归方程为Y=8 98.7X+47.9,r=0.999 9,结果表明马钱苷酸在11.563~185.000μg/m L范围内线性关系良好。龙胆苦苷含量均值依次为拉萨>林芝>昌都,马钱苷酸含量均值依次为拉萨>昌都>林芝;且西藏8个产地中只有昌都地区一个样地的长梗秦艽中龙胆苦苷与马钱苷酸的含量总和未达到2015版《中华人民共和国药典》标准(2.5%)。结论该方法快捷、有效,可用于长梗秦艽中龙胆苦苷与马钱苷酸的含量测定。
Objective To compare the difference of component contents of Gentiana waltonii from different a reas in Tibet. Methods Eight samples of Gentiana waltonii were collected from 3 regions in Tibet. The contents of gentiopicroside and loganin acid were determined by HPLC. The samples were separated on the column of Kromasil C18( 4.6 mm×250 mm,5 μm),which moving phase was methanol-0.1% phosphoric acid solution as well as gradient elution was used. The flow rate was 1 m L / min. The detected wave-length was at 245 nm. The column temperature was at 35 ℃ and the sample size was 10 μL. Results The regression equation of gentiopicroside was Y = 1 050 X +34.6,r = 0.999 7,showing a good linearity in the range of 61.063-977.000 μg / m L; the regression e quation of loganic acid was Y = 898.7X +47.9,r = 0.999 9,showing a good linearity in the range of 11.563-1 85.000 μg / m L.The contents of gentiopicroside in three regions showed as Lhasa>Nyingchi>Chamdo and that of loganic acid showed as Lhasa>Chamdo>Nyingchi. Among the 8 producing areas in Tibet,the total content of g entiopicroside and loganin acid in Gentiana waltonii only from Chamdo was under 2. 5%( specified standard in C hinese Pharmacopoeia).Conclusion This method is simple and accurate,which can be used to determine the g entiopicroside and loganic acid in Gentiana waltonii.