文献类型：期刊文献

英文题名：PEGylated NALC-functionalized gold nanoparticles for colorimetric discrimination of chiral tyrosine

作者：Chen, Xin-Yue[1,2];Ha, Wei[1,2];Jin, Xiao-Jie[3];Shi, Yan-Ping[1,2]

第一作者：Chen, Xin-Yue

通信作者：Ha, W[1];Shi, YP[1];Ha, W[2];Shi, YP[2]

机构：[1]Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China;[2]corresponding author), Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China.

年份：2020

卷号：145

期号：22

起止页码：7397

外文期刊名：ANALYST

收录：;EI(收录号：20204609496547);Scopus(收录号：2-s2.0-85095861957);WOS:【SCI-EXPANDED(收录号:WOS:000587582800028)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 21405164 and 21974145) and the Youth Innovation Promotion Association (2017457) of the Chinese Academy of Sciences.

语种：英文

外文关键词：Acetylcysteine - Amino acids - Color - Enantiomers - Fiber optic sensors - Gold nanoparticles - Metal nanoparticles

摘要：In this work, acid and matrix-tolerant multifunctionalized gold nanoparticles (AuNPs) with an integrated chiral selector towards tyrosine (Tyr) and polyethylenglycol (PEG) chains were developed for visual chiral discrimination of Tyr in biological samples under acid conditions. In brief, AuNPs multifunctionalized with N-acetyl-l-cysteine (NALC) and PEG (PEG/NALC-AuNPs) were prepared via a simple strategy. In the presence of l-Tyr, the color of PEG/NALC-AuNP solution changed from red to gray, while no obvious color change was observed with the introduction of d-Tyr, which indicated that the introduction of PEG onto the surface of AuNPs has no effect on the chiral recognition between l-Tyr and NALC. A computer-aided molecular model was used to clarify the chiral recognition mechanism between NALC and Tyr enantiomers and to further guide the optimization of sensitivity. The resultant PEG/NALC-AuNP sensor presented a significantly improved stability under acid and alkali conditions compared with conventional NALC-AuNPs, resulting in a wider dynamic range (500 nM-100 mu M) and a 50 times reduced detection limit by simply adjusting the pH of the sensor system under acid conditions (pH 2-2.5). More importantly, the PEG/NALC-AuNPs can realize the visual chiral discrimination of Tyr enantiomers in biological samples due to their significantly improved long-term stability and reduced interaction towards non-target species.