文献类型：期刊文献

中文题名：当归贝母苦参丸含药血清对胃癌细胞SGC-7901侵袭转移能力和周期的影响

英文题名：Effects of Angelica Flavescens Fritillaria Pill Medicated Serum on Cell Cycle Invasion and Metastasis of Gastric Cancer Cell Lines SGC-7901

作者：师金凤[1];李海龙[1,2];王红蕾[2];邓建男[2];杨植媛[2];吴红彦[1,2]

第一作者：师金凤

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：16

期号：10

起止页码：30

中文期刊名：辽宁中医药大学学报

外文期刊名：Journal of Liaoning University of Traditional Chinese Medicine

收录：CSTPCD

基金：甘肃省高等学校科研项目(2013A-088);甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2012-003)

语种：中文

中文关键词：当归贝母苦参丸;含药血清;胃癌;细胞周期;侵袭转移

外文关键词：Angelica Flavescens Fritillaria Pill; medicated serum; gastric cancer; cell cycle; invasion and metastasis

摘要：目的：探讨不同浓度的当归贝母苦参丸含药血清抑制胃癌SGC-7901、MKN-45细胞增殖作用及其相关机制。方法：不同浓度含药血清处理SGC-7901细胞后,（1）MTT法测定细胞活力;（2）流式细胞术检测细胞周期;（3）Transwell侵袭实验及划痕实验观察侵袭能力和转移能力。结果：不同浓度的当归贝母苦参丸含药血清均能抑制细胞增殖,在10%浓度时抑制作用达最大值;侵袭及划痕实验示：含药血清干预后细胞侵袭及转移能力减弱;流式细胞术检测显示：含药血清干预后细胞S期比例下降,G0~G1期比例上升。结论：当归贝母苦参丸含药血清可以抑制SGC-7901细胞增殖,减弱其侵袭转移能力,其机制可能与其干预细胞周期有关。
Objective： To investigate the effects of different concentrations of Angelica Flavescens Fritillaria Pill medicated serum on cell cycle, invasion and metastasis of gastric cancer cell lines SGC-7901 with its related mechanisms. Methods ： After treatment on gastric cancer cell lines SGC-7901 with different concentrations of Angelica Flavescens Fritillaria Pill medicated serum, the following methods were used： （ 1 ）MTT was employed to test the vialibity of gastric cancer cell lines SGC-7901. （2）The flow eytometry was used to detect cell cycle. （ 3 ）Transwell and scratch adhesion test was used to detect the invasion and metastasis abilities. Results ： Angelica Flavescens Fritillaria Pill medicated serum of different concentrations can inhibit the proliferation of SGC-7901 cell lines, and medicated serum of 10% concentration reached a maximum inhibition. Transwell and scratch adhesion test showed that medicated serum could reduce migration and metastasis of gastric cancer cell lines SGC-7901. The flow cytometry analysis showed medicated serum could shorten S phase of cells, and increase G0-G1 phase. Conclusion ： Angelica Flavescens Fritillaria Pill medicated serum could inhibit the proliferation of SGC-7901 cell lines, weakening its invasion and migration capacity and the mechanisms of which may be related to the intervention of cell cycle.