文献类型：期刊文献

英文题名：Inactivation of the Tuberomammillary Nucleus by GABA_A Receptor Agonist Promotes Slow Wave Sleep in Freely Moving Rats and Histamine-Treated Rats

作者：Xie, Jun-Fan[1];Fan, Kun[1];Wang, Can[1];Xie, Peng[1];Hou, Min[1,2];Xin, Le[1];Cui, Guang-Fu[1];Wang, Lin-Xin[1];Shao, Yu-Feng[1];Hou, Yi-Ping[1]

第一作者：Xie, Jun-Fan

通信作者：Shao, YF[1];Hou, YP[1]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Dept Neurosci Anat Histol & Embryol, 199 Donggang Xi Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Sch Basic Med Sci, Dept Anat Histol & Embryol, 35 Dingxi Dong Rd, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Dept Neurosci Anat Histol & Embryol, 199 Donggang Xi Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Dept Neurosci Anat Histol & Embryol, 199 Donggang Xi Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2017

卷号：42

期号：8

起止页码：2314

外文期刊名：NEUROCHEMICAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000406287900018)】；

基金：This work was supported by the National Natural Science Foundation of China (30670677, 81071076, 81171254 and 81471347 to YPH; 31500853 to YFS) and the Fundamental Research Funds for the Central University (lzujbky-2015-167 and lzujbky-2015-277 to YFS). The authors declare no conflicts of interest in this manuscript.

语种：英文

外文关键词：Histamine; GABA; Tuberomammillary nucleus; Sleep-wake states; EEG power spectral density

摘要：A prominent hypothesis, the "flip-flop switch" model, predicts that histaminergic (HAergic) neurons in the tuberomammillary nucleus (TMN), an important component of the ascending arousal system, are inactivated by GABA mainly from the ventrolateral preoptic nucleus to allow the appearance and maintenance of sleep. However, which sleep state and the band of EEG activity induced by GABAergic inactivation of the TMN are unclear. In this study, alterations of sleep-wake states and cortical EEG power spectral density were investigated following muscimol, a GABA(A)-receptor agonist, microinjected bilaterally into the TMN in freely moving rats and HA pretreated rats, respectively. Muscimol dosed at 0.25 and 0.50 mu g/side into the TMN during dark period dose-dependently increased slow wave sleep (SWS) accompanied by an increase in cortical EEG delta (0.5-4 Hz) and spindle (8.2-12 Hz) activities. In the meanwhile, wakefulness and EEG beta (12.2-30 Hz) activity were decreased significantly, while paradoxical sleep and EEG theta (4.2-8 Hz) activity were not changed. The increase of muscimol-induced SWS was because of prolonged SWS bout duration and not to an increased bout number. Muscimol (0.50 mu g/side) administration 2 h after HA (0.125 mu g/side) treatment during light period reversed the HA-induced wakefulness and EEG beta 2 (20.2-30 Hz) activity into SWS and EEG delta activity. These results demonstrate that the GABAergic inactivation of the TMN in freely moving rats and HA-treated rats promotes SWS and slow activity of cortical EEG, suggesting that the potential function of the GABA(A) receptor in the TMN is to dampen vigilant arousal.