文献类型：期刊文献

英文题名：Concentration Change Study of Five Bioactive Components in Rat Plasma of Hedysari Radix with Before and after Rubbing-Processed by Uplc-Ms/Ms Method

作者：Luo, Xudong[1]; Li, Chengyi[1]; Qi, Peng[1]; Liang, Tingting[1]; Li, Bo[1]; Feng, Xiaoli[1]; Wang, Mingwei[1]; Liu, Shubin[1]; Qiang, Zhengze[1]; Wang, Yan[1]; Jia, Miaoting[1]; Li, Xu[1]; He, Jungang[1]; Wei, Xiaocheng[1]

第一作者：Luo, Xudong

机构：[1] Laboratory for Identification and Quality Evaluation of Chinese Medicinal Herbs, Department of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230200377)

语种：英文

外文关键词：Medicine - Rats

摘要：Hedysari Radix is a commonly used traditional Chinese medicine that improves immunity; formononetin, ononin, calycosin, medicarpin, and vanillic acid play an important role in achieving this effect. Herein, a precise and sensitive UPLC MS/MS method was developed and validated for the determination of formononetin, ononin, calycosin, medicarpin, and vanillic acid in rat plasma after administering doses of rubbing- and non-rubbing-processed Hedysari Radix. The five bioactive components were extracted from plasma using a protein precipitation method as well as an internal standard method (IS, pioglitazone) to quantify the five bioactive components. A Waters CORTES C18 (4.6 mm×50 mm, 2.7 μm) and a mobile phase composed of methanol and water (containing 0.2% formic acid) were used for the separation. The method was linear within the concentration range of 19.53 to 625.00 ng/mL for formononetin; 0.01 to 3.13 ng/mL for ononin; 0.01 to 3.13 ng/mL for calycosin; 0.16 to 5.00 ng/mL for medicarpin; and 19.53 to 625.00 ng/mL for vanillic acid. This method has a low lower limit of quantification, is simple, and has a short analysis time and a high degree of separation. The intra- and inter-day precisions of QC samples were found to be below 8.58 and 12.64%, respectively. Accuracy ranged from -8.61 to 10%. Finally, the developed method was used to determine the concentrations of the five bioactive components in rat plasma after the administration of rubbing- and non-rubbing processed Hedysari Radix. ? 2023, The Authors. All rights reserved.