文献类型：期刊文献

中文题名：基于高通量测序的当归抽薹相关基因分析

英文题名：Mining of Premature Bolting-related Genes of Angelica sinensis Based on High-throughput Sequencing

作者：王振恒[1,2,3];王引权[1,2,3];雒军[1,2];荔淑楠[1];晋玲[1,2,3];陈燕[1]

第一作者：王振恒

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000;[3]西北中藏药协同创新中心,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：24

期号：2

起止页码：243

中文期刊名：中国现代中药

外文期刊名：Modern Chinese Medicine

收录：CSTPCD

基金：国家重点研发计划项目(2017YFC1700705);甘肃省教育厅双一流重大科研项目(GSSYLXM-05);2019年甘肃中医药大学扶贫科技专项(2019FPZX-1)。

语种：中文

中文关键词：当归;抽薹;转录组;差异表达基因

外文关键词：Angelica sinensis(Oliv.)Diel;bolting;transcriptome;differentially expressed genes

摘要：目的:筛选与当归抽薹相关的基因并探讨其分子机制。方法:取抽薹和未抽薹当归植株,剪取倒数第三片功能叶中部小叶片,利用BGISEQ-500测序平台对当归叶片基因进行高通量测序,再通过非冗余蛋白(NR)、核酸序列(NT)、Swiss-Prot、InterPro、京都基因与基因组百科全书(KEGG)、真核生物蛋白相邻类的聚簇(KOG)、基因本体(GO)数据库进行功能基因注释。结果:抽薹当归与未抽薹当归共有936个差异表达基因(DEGs),在公共数据库比对得到867个DEGs,其中505个基因有明确功能,这些功能基因中有182个上调基因。在DEGs中筛选与当归抽薹相关的基因。其中调节细胞生长的基因有CDC48C、CSLA2、CYCA1-1、AUG8、CER26、At1g65390、BTAF1;调节开花的基因有TPS1、ALA6、AP2、SOCI;调节叶片光合作用的基因主要有CAB37、CAP10A。结论:细胞形态建成相关的基因在抽薹当归叶的生长发育过程中发挥了重要作用。抽薹和未抽薹当归差异表达基因的研究丰富了当归基因资源,为进一步开展分子生物学及分子育种研究提供了基础数据。
Objective:To mine the genes involved in the bolting of Angelica sinensis(Oliv.) Diel and explore the underlying molecular mechanism.Methods:The central leaflet of the third functional leaf from the top of the A.sinensis plants which underwent and did not undergo bolting was collected for high-throughput sequencing on the BGISEQ-500 platform.Unigenes were annotated in Non-Redundant Protein Sequence Database(NR),Nucleotide Sequence Database(NT),Swiss-Prot,Kyoto Encyclopedia of Genes and Genomes(KEGG),EuKaryotic Orthologous Groups(KOG),Interpro and Gene Ontology(GO).Results:A total of936 differentially expressed genes(DEGs) were identified,among which 867 genes can be identified in public databases.Among the identified genes,505 had known functions,including 182 upregulated genes.The DEGs related to premature bolting were screened out.Specifically,CDC48 C,CSLA2,CYCA1-1,AUG8,CER26,At1 g65390 and BTAF1 can regulate cell growth;TPS1,ALA6,AP2 and SOCI were involved in flowering;CAB37 and CAP10 A were the main genes regulating photosynthesis.Conclusion:The genes involved in cell morphogenesis play a role in the bolting of A.sinensis.The study of DEGs between bolting and control group of A.sinensis enriches the gene resources and provides basic data for further research of molecular biology and molecular breeding.